Abstract
The collection of analyte zones in capillary electrophoresis which are separated with high efficiency allows the identification and characterization of the analytes by analytical techniques which cannot be directly connected to CE. One common design of a fraction collector in CE is based on the replacement of the outlet buffer vial with collection vials, which can be achieved by modification of commercial instruments. This arrangement often gives inexact collection and, in the cases where the collection vials contain a small volume of buffer, gives dilution of the fraction. The first problem is solved by calculating the migration time for fraction collection corresponding to the related migration behavior [1].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Lee, H.G.; Desiderio, D.M. (1994) Preparative capillary zone electrophoresis of synthetic peptides. Conversion of an autosampler into a fraction collector. J. Chromatogr. 686, 309–317.
Cheng, Y-F.; Fuchs, M.; Andrews, D.; Carson, W. (1992) Membrane fraction collection for capillary electrophoresis. J. Chromatogr. 608, 109–116.
Fujimoto, C.; Fujikawa, T.; Jinno, K. (1992) Sample collection by a capillary zone electrophoretic system with an on-column fracture. J. High Resolut. Chromatogr. 15, 201–203.
Müller, O.; Foret, F.; Karger, B.L. (1995) Design of a high-precision fraction collector for capillary electrophoresis. Anal. Chem. 67, 2974–2980.
Heiger, D.N.; Cohen, A.S.; Karger, B.L. (1990) Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields. J. Chromatogr. 516, 33–48.
Sudor, J.; Novotny, M. (1993) Electromigration behavior of polysaccharides in capillary electrophoresis under pulsed-field conditions. Proc. Natl Acad. Sci. USA 90, 9451–9455.
Sudor, J.; Novotny, M.V. (1994) Pulsed-field capillary electrophoresis: Optimizing separation parameters with model mixtures of sulfonated polystyrenes. Anal. Chem. 66, 2139–2147.
Heiger, D.N.; Carson, S.M.; Cohen, A.S.; Karger, B.L. (1992) Wave form fidelity in pulsed-field capillary electrophoresis. Anal. Chem. 64, 192–199.
Demana, T.; Guhathakurta, U.; Morris, M.D. (1992) Effects of analyte velocity modulation on the electroosmotic flow in capillary electrophoresis. Anal. Chem. 64, 390–394.
Heinrich, J.; Wagner, H. (1992) High speed electrophoresis simulation for optimization of continuous flow electrophoresis and high performance capillary techniques: Part I. Computer model. Electrophoresis 13, 44–49.
Reijenga, J.C.; Kenndler, E. (1994) Computational simulation of migration and dispersion in free capillary zone electrophoresis: I. Description of the theoretical model. J. Chromatogr. 659, 403–415.
Reijenga, J.C.; Kenndler, E. (1994) Computational simulation of migration and dispersion in free capillary zone electrophoresis: II. Results of simulation and comparison with measurements. J. Chromatogr. 659, 417–426.
Ermakov, S.V.; Bello, M.S.; Righetti, P.G. (1994) Numerical algorithms for capillary electrophoresis. J. Chromatogr. 661, 265–278.
Knox, J.H.; McCormack, K.A. (1994) Volume expansion and loss of sample due to initial self-heating in capillary electroseparation (CES) systems. Chromatographia 38, 279–282.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Chapman & Hall
About this chapter
Cite this chapter
Kitagishi, K. (1997). Other systems. In: Shintani, H., Polonský, J. (eds) Handbook of Capillary Electrophoresis Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1561-9_9
Download citation
DOI: https://doi.org/10.1007/978-94-009-1561-9_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7197-0
Online ISBN: 978-94-009-1561-9
eBook Packages: Springer Book Archive