Epithelial electrophysiology

  • Simon A. Lewis


At the beginning of this book we categorized epithelia as being either leaky (low electrical resistance) or tight (high electrical resistance). Chapter 4 provides a description of and uses for transepithelial electrical measurements. It is the purpose of this chapter to describe in detail the methods which allow one to characterize the electrical properties of an epithelium.


Apical Membrane Basolateral Membrane Voltage Response Transepithelial Resistance Agar Bridge 
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  1. Crowe, W.E. and Wills, N.K. (1991) A simple method for monitoring changes in cell height using fluorescent microbeads and a Ussing-type chamber for the inverted microscope. Pflügers Arch. 419:349-57.PubMedCrossRefGoogle Scholar
  2. Frömter, E. and Gebier, B. (1977) Electrical properties of amphibian urinary bladder epithelia. Pflügers Arch. 371:99-108.PubMedCrossRefGoogle Scholar
  3. Lewis, S.A., Eaton, DC, Clausen, C. and Diamond, J.M. (1977) Nystatin as a probe for investigating the electrical properties of a tight epithelium. J. Gen. Physiol. 70:427-40.PubMedCrossRefGoogle Scholar
  4. Lewis, S.A. and Wills, N.K. (1980) Resistive artifacts in liquid-ion exchanger microelectrodes estimates of Na+ activity in epithelial cells. Biophys. J. 31:127-38.PubMedCrossRefGoogle Scholar
  5. Lewis, S.A. and Wills, N.K. (1982) Electrical properties of the rabbit urinary bladder assessed using gramicidin D. J. Memb. Biol. 67:45-53.CrossRefGoogle Scholar
  6. Wills, N.K., Lewis, S.A. and Eaton, D.C. (1978) Active and passive properties of rabbit descending colon: a microelectrode and nystatin study. J. Membr. Biol. 45:81-108.Google Scholar
  7. Yonath, J. and Civan, M.M. (1971) Determination of the driving force of the Na+ pump in toad bladder by means of vasopressin. J. Memb. Biol. 5:366-85.CrossRefGoogle Scholar

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© Chapman & Hall 1996

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  • Simon A. Lewis

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