Abstract
Low molecular weight plasmids carried by pharyngeal isolates of Neisseria meningitidis and Neisseria lactamica have been tested using DNA probe hybridisation. Homology has been demonstrated between the gonococcal cryptic plasmid and a group of plasmids of approximately 2.8 Megadaltons (MD) which were carried by 11/16 strains of meningococci and 2/6 strains of Neisseria lactamica tested. A group of plasmids of < 1.6MD showed no homology to the cryptic plasmid. A plasmid isolated from meningococci (strain 222) of < 1.6MD was shown to hybridise with a plasmid carried by 6 strains of meningococci and one strain of N. lactamica. All strains also carried the 2.8MD plasmid. A plasmid isolated from meningococci (strain 388), also of < 1.6MD did not hybridise with any other plasmid.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bhatti, A. R., K. O’Hara, L. A. White & L. E. Bryan (1981) Presence of plasmid deoxyribonucleic acid inNeisseria meningitidis. FEMS Microbiology Letters, 10: 175–177
Birnboim, H. C. & J. Doly (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research. 7: 1513–1523
Ison, C. A., C. M. Bellinger & A. A. Glynn (1984) Plasmids in throat and genital isolates of meningococci. J. Clin. Pathol. 37: 1123–1128
Ison, C. A., C. M. Bellinger & C. S. F. Easmon (1985) Plasmids in meningococci. In: The PathogenicNeisseriae(pp. 216–218) G. K. Schoolnik (Ed). American Society for Microbiology, Washington D.C
Ison, C. A., C. M. Bellinger & J. Walker (1986) Homology of the cryptic plasmid of Neisseria gonorrhoeae with plasmids fromNeisseria meningitidisandNeisseria lactamica. J. Clin. Pathol. 39: 1119–1123
Maniatis, T., T. F. Fritisch & J. Sambrook (1982) Molecular cloning: A Laboratory Manual. Cold Spring Harbour, New York
Mazloum, H., P. A. Totten, G. F. Brooks, C. R. Dawson, S. Falkow, J. F. James, J. S. Knapp, J. M. Koomey, C. J. Lammel, D. Peters, J. Schacter, W. S. Tang & N. A. Vedros (1986) An unusualNeisseriaisolated from conjuctival cultures in rural Egypt. J. Infect. Dis. 154: 212–224
Totten, P. A., K. K. Holmes, H. H. Handsfield, J. S. Knapp, P. L. Perine & S. Falkow (1983) DNA hybridisation technique for the detection ofNeisseria gonorrhoeaein men with urethritis. J. Infect. Dis. 148: 462–71
Verschueren, H., M. Dekegel, D. Dekegel, C. Gilquin & S. DeMayer (1982) Plasmids inNeisseria meningitidis. Lancet i: 851851–852
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Kluwer Academic Publisher, Dordrecht
About this chapter
Cite this chapter
Ison, C.A., Bellinger, C.M., Gedney, J., Walker, J. (1988). DNA hybridisation between the gonococcal cryptic plasmid and plasmids isolated from Neisseria meningitidis and Neisseria lactamica . In: Poolman, J.T. (eds) Gonococci and Meningococci. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1383-7_19
Download citation
DOI: https://doi.org/10.1007/978-94-009-1383-7_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7117-8
Online ISBN: 978-94-009-1383-7
eBook Packages: Springer Book Archive