Energy Metabolism and Transport in Neonatal Heart Cells in Culture

  • A. Pinson
  • T. Huizer
Part of the Developments in Cardiovascular Medicine book series (DICM, volume 91)

Abstract

Heart cells in culture are increasingly being used in studies at the cellular level in many fields of cardiac biology and pathology. This chapter reviews the metabolism of the energy-producing substrates - glucose and fatty acids (FA’s) - pinpointing the findings in cultured heart cells and their contribution to understand uptake and subsequent distribution of these metabolites. The roles of glucose and FA’s in energy production and balance have been determined mainly from studies of 14CO2 production from labelled substrates. Oxidation of glucose and FA’s is incomplete if either of these substrates is present in excess, indicating that substrate utilization is controlled by a cellular mechanism. In addition, FA’s in cellular reserves seem to be preferentially utilized over newly incorporated ones, implying that cellular lipids undergo continuous renewal The recently developed technique for measuring oxygen consumption without disrupting the cultures will undoubtedly allow further progress to be made in elucidating the energy demands of the cardiomyocyte. The regulation of high-energy phosphate production and its transport from the mitochondria to the myofibrils via the creatine kinase system are also discussed in this chapter. Cultured heart cells have been used for studying oxygen deprivation. Under suitable conditions, such systems provide information on the sequence of events at the cellular level during anoxia.

Keywords

Creatine Kinase Erucic Acid Glucose Oxidation Citric Acid Cycle Heart Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • A. Pinson
    • 1
  • T. Huizer
    • 1
    • 2
  1. 1.Laboratory for Myocardial ResearchHebrew University-Hadassah Medical SchoolJerusalemIsrael
  2. 2.Cardiochemical LaboratoryThoraxcenterRotterdamThe Netherlands

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