The Nature of an Ethylene Biosynthesis-Inducing Factor Found in Cellulysin
The proteinaceous ethylene biosynthsis-inducin factor (EIF) that was isolated from Cellulysin was shown to contain a xylanase activity. In all non-denaturing protein separation methods employed (Sephacryl S-200 chromatography, preparative isoelectric focusing and agarose electrophoresis), xylanase activity co-purified with the ethylene biosynthesis-inducing activity. Treatment with heat (60°C) or proteases in 8 M urea inhibited both ethylen biosynthesis-inducing and xylanase activities. Antibodies raised against EIF immunoprecipitated both ethylene biosynyhesis-inducing and xylanase activities. The purified EIF contained no detectable cellulase, polygalacturonase, protease or other hydrolytic activities as estimated by using p-nitrophenyl derivatives of several sugars as substrates. Based on these data, we suggest that EIF contains a specific xylanase activity which is involved in inducing ethylene biosynthesis.
KeywordsXylanase Activity Ethylene Biosynthesis Birch Wood Xylan Pear Fruit Agarose Electrophoresis
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