Hepatic Bile Acid Transport and Secretion

  • S. Erlinger


Bile acid transport and secretion by the liver is one of the important steps in the recirculation of bile acid within the body. After bile acids return from the intestine, they have first to be taken up by the parenchymal liver cell, then transported from the sinusoidal pole to the canalicular pole of the cell, and finally secreted into the bile canalicular lumen. In addition, unconjugated bile acids have to be conjugated, chiefly with taurine or glycine, before secretion. The purpose of this review is to examine the cellular mechanisms of bile acid transport by the liver, with particular emphasis on intracellular transport, the step which is at present least well understood.


Bile Acid Bile Acid Transport Unconjugated Bile Acid Canalicular Secretion Bile Acid Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Glasinovic, J.C., Dumont, M., Duval, M. and Erlinger, S. (1975). Hepatocellular uptake of taurocholate in the dog. J. Clin. Invest., 55, 419–26CrossRefGoogle Scholar
  2. 2.
    Glasinovic, J.C., Dumont, M., Duval, M. and Erlinger, S. (1975). Hepatocellular uptake of bile acids in the dog: Evidence for a common carrier-mediated transport system. An indicator dilution study. Gastroenterology, 69, 973–81PubMedGoogle Scholar
  3. 3.
    Reichen, J. and Paumgartner, G. (1975). Kinetics of taurocholate uptake by the perfused rat liver. Gastroenterology, 68, 132–6PubMedGoogle Scholar
  4. 4.
    Reichen, J. and Paumgartner, G. (1976). Uptake of bile acids by the perfused rat liver. Am. J. Physiol., 231, 734–42PubMedGoogle Scholar
  5. 5.
    Anwer, M.S., Kroker, R. and Hegner, D. (1976). Cholic acid uptake into isolated rat hepatocytes. Hoppe Seylefs Z’. Physiol. Chem., 357, 1477–86CrossRefGoogle Scholar
  6. 6.
    Scharschmidt, B.F. and Stephens, J.E. (1981). Transport of sodium, chloride, and taurocholate by cultured rat hepatocytes. Proc. Natl. Acad. Sci. USA, 78, 986–90PubMedCrossRefGoogle Scholar
  7. 7.
    Schwarz, L.R., Burr, R., Schwenk, M., Pfaff, E. and Greim, H. (1975). Uptake of taurocholic acid into isolated rat-liver cells. Eur. J. Biochem., 55, 617–23PubMedCrossRefGoogle Scholar
  8. 8.
    Paumgartner, G. and Reichen, J. (1975). Different pathways for hepatic uptake of taurocholate and indocyanine green. Experientia, 31, 306–7PubMedCrossRefGoogle Scholar
  9. 9.
    Blitzer, B.L. and Donovan, C.B. (1984). A new method for the rapid isolation of basolateral plasma membrane vesicles from rat liver. Characterisation, validation, and bile acid transport studies. J. Biol. Chem., 259, 9295–301PubMedGoogle Scholar
  10. 10.
    Duffy, M.C., Blitzer, B.L. and Boyer, J.L. (1983). Direct determination of the driving forces for taurocholate uptake into rat liver plasma membrane vesicles. J. Clin. Invest., 72, 1470–81PubMedCrossRefGoogle Scholar
  11. 11.
    Inoue, M., Kinne, R., Tran, T. and Arias, I.M. (1982). Taurocholate transport by rat liver sinusoidal membrane vesicles: Evidence of sodium cotransport. Hepatology, 2, 572–9PubMedCrossRefGoogle Scholar
  12. 12.
    Ruifrok, P.G. and Meijer, D.K.F. (1982). Sodium ion-coupled uptake of taurocholate by rat-liver plasma membrane vesicles. Liver, 2, 28–34PubMedGoogle Scholar
  13. 13.
    Wieland, T., Nassal, M., Kramer, W., Fricker, G., Bickel, U. and Kurz, G. (1984). Identity of hepatic membrane transport systems for bile salts, phalloidin, and antamanide by photoaffinity labeling. Proc. Natl. Acad. Sci. USA, 81, 5232–6PubMedCrossRefGoogle Scholar
  14. 14.
    von Dippe, P. and Levy D. (1983). Characterisation of the bile acid transport system in normal and transformed hepatocytes. J. Biol. Chem., 258, 8896–901Google Scholar
  15. 15.
    von Dippe, P., Ananthanarayanan, M., Drain, P. and Levy, D. (1986). Purification and reconstitution of the bile acid transport system from hepatocyte sinusoidal plasma membranes. Biochim. Biophys. Acta, 862, 352–60CrossRefGoogle Scholar
  16. 16.
    Strange, R.C., Cramb, R., Hayes, J.D. and Percy-Robb, I.W. (1977). Partial purification of two lithocholic acid-binding proteins from rat liver 100,000 g supernatants. Biochem. J., 165, 425–9PubMedGoogle Scholar
  17. 17.
    Strange, R.C., Nimmo, I.A. and Percy-Robb, I.W. (1977). Binding of bile acids by 100,000 g supernatants of rat liver. Biochem. J., 162, 659–64PubMedGoogle Scholar
  18. 18.
    Stolz, A., Sugiyama, Y., Kuhlenkamp, J. and Kaplowitz, N. (1984). Identification and purification of a 36 kDa bile acid binder in human hepatic cytosol. FEBS Lett., 177, 31–5PubMedCrossRefGoogle Scholar
  19. 19.
    Sugiyama, U., Yamada, T. and Kaplowitz, N. (1983). Newly identified bile acid binders in rat liver cytosol. Purification and comparison with glutathione S-transferases. J. Biol. Chem., 258, 3602–7PubMedGoogle Scholar
  20. 20.
    Erlinger, S., Dumont, M., Zouboulis-Vafiadis, I. and De Couët, G. (1984). The importance of conjugation in biliary secretion of ursodeoxycholate and 7-ketolithocholate in the rat. Clin. Sci., 487–91Google Scholar
  21. 21.
    Vessey, D.A., Whitney, J. and Gollan, J.L. (1983). The role of conjugation reactions in enhancing biliary secretion of bile acids. Biochem. J., 214, 923–7PubMedGoogle Scholar
  22. 22.
    Zouboulis-Vafiadis, I., Dumont, M. and Erlinger, S. (1982). Conjugation is rate limiting in hepatic transport of ursodeoxycholate in the rat. Am.J. Physiol., 243, G208–13PubMedGoogle Scholar
  23. 23.
    Lamri, Y., Roda, A., Dumont, M., Feldmann, G. and Erlinger, S. (1987). Immunoperoxidase localisation of bile salts in rat liver cells. J. Hepatol., 5, 539 (abstract)Google Scholar
  24. 24.
    Groothuis, G.M.M., Hardonk, M J., Keulemans, K.P.T., Nieuwenhuis, P. and Meijer, D.K.F. (1982). Autoradiographic and kinetic demonstration of acinar heterogeneity of taurocholate transport. Am. I. Physiol., 243, G455–62Google Scholar
  25. 25.
    Suchy, FJ., Balistreri, W.F., Hung, J., Miller, P. and Garfield, S.A. (1983). Intracellular bile acid transport in rat liver as visualised by electron microscope autoradiography using a bile acid analogue. Am. J. Physiol., 245, G681–9PubMedGoogle Scholar
  26. 26.
    Goldsmith, M.A., Huling, S. and Jones, A.L. (1983). Hepatic handling of bile salts and protein in the rat during intrahepatic cholestasis. Gastroenterology, 84, 978–86PubMedGoogle Scholar
  27. 27.
    Simion, A.F., Fleischer, B. and Fleischer, S. (1984). Two distinct mechanisms for taurocholate uptake in subcellular fractions from rat liver. J. Biol Chem., 259, 10814–22PubMedGoogle Scholar
  28. 28.
    Dubin, M., Maurice, M., Feldmann, G. and Erlinger, S. (1980). Influence of colchicine and phalloidin on bile secretion and hepatic ultrastructure in the rat. Possible interaction between microtubules and microfilaments. Gastroenterology, 79, 646–54PubMedGoogle Scholar
  29. 29.
    Inoue, P., Kinne, R., Tran, T. and Arias, I.M. (1984). Taurocholate transport by rat liver canalicular membrane vesicles. Evidence for the presence of an Na +-independent transport system. J. Clin. Invest., 73, 659–63PubMedCrossRefGoogle Scholar
  30. 30.
    Meier, P.J., St. Meier-Abt, A., Barrett, C., and Boyer, J.L. (1984). Mechanisms of taurocholate transport in canalicular and basolateral rat liver plasma membrane vesicles. J. Biol Chem., 259, 10614–22PubMedGoogle Scholar
  31. 31.
    Ruetz, S., Fricker, G., Hugentobler, G., Winterhalter, K., Kurz, G. and Meier, P.J. (1987). Isolation and characterisation of the putative canalicular bile salt transport system of rat liver. J. Biol Chem., 269, 11324–30Google Scholar
  32. 32.
    Ruetz, S., Hugentobler, G. and Meier, P.J. (1987). Functional reconstitution of the canalicular bile salt transport system of rat liver. Hepatology, 7, 1105Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • S. Erlinger
    • 1
  1. 1.Unité de Recherches de Physiopathologie HépatiqueClichy CedexFrance

Personalised recommendations