Antigenic Phenotype and Experimental Herpes Simplex Virus Infection of Cultured Human Fetal Neural Cells
Human neural cell cultures have proved to be a useful tool in studying the developmental biology of the human nervous system1, 2. These tissue culture systems have also been used in a variety of ways to investigate the pathogenesis of various neurological diseases in which immunopathological mechanisms may be important3–5 and the susceptibility of human neural cells to infection with viruses6, 7. We have favoured the use of cultured human fetal neural cells in such studies because of their greater relevance to human disease compared with cell cultures derived from animal tissues, and also in view of the obvious ethical contraindications to studying such cells in vivo.
KeywordsSchwann Cell Herpes Simplex Virus Type Herpes Simplex Virus Encephalitis Tetanus Toxin Antigenic Phenotype
Unable to display preview. Download preview PDF.
- 10.Dickson, J. G., Flanigan, T. P. and Walsh, F. S. (1982). Cell-surface antigens of human fetal brain and dorsal root ganglion cells in tissue culture. In Rowland, L. P. (ed.). Human Motor Neuron Diseases. pp. 435–51. ( New York: Raven Press )Google Scholar
- 15.Cotmore, S. F., Crowhurst, S. A. and Waterfield, M. D. (1981). Purification of Thy-1 related glycoproteins from human brain and fibroblasts: comparisons between these molecules and murine glycoproteins carrying Thy-1 and Thy-1,2. Eur. J. Biochem., 11, 597–603Google Scholar
- 38.Johnson, R. T. (1982). Viral Infections of the Nervous System. ( New York: Raven Press )Google Scholar
- 39.Bartinger, J. R. (1975). Herpes simplex virus infection of nervous tissue in animals and man. Progr. Med. Virol., 20, 1–26Google Scholar
- 41.Brown, S. M., Ritchie, D. A. and Subak-Sharpe, J. H. (1973). Genetic studies with herpes simplex virus type 1. The isolation of temperature sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. J. Gen. Virol., 18, 329–46PubMedCrossRefGoogle Scholar
- 42.Clements, G. B. (1975). Selection of biochemically variant, in some cases mutant, mammalian cells in culture. In Klein, G. and Weinhouse, S. (eds.) Advances in Cancer Research. pp. 274–380. ( New York and London: Academic Press )Google Scholar
- 43.Subak-Sharpe, J. H. (1973). The genetics of herpes virus. Cancer Res., 32, 1385–92Google Scholar
- 47.Booss, J. and Esiri, M. M. (1986). Viral Encephalitis. Pathology, Diagnosis and Management. ( Oxford: Blackwell Scientific Publications )Google Scholar