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Observability by pH in Bioprocesses

  • M. N. Pons
  • L. Garrido-Sanchez
  • J. M. Engasser

Abstract

The shortage of direct sensors in bioprocesses is a crucial problem which restrains their automation. It is therefore important to use at the best those which are already available. Among them the pH probe is an essential instrument, despite its somewhat debatable reliability, and pH measurements are a routine task. Two cases should be examined: when pH is not regulated, its variation can be related to biomass or secondary metabolite variation as in acetic fermentation; when pH is regulated, one looks for a global relation between the consumption of the neutralization reagent and the biomass or metabolite production as in the aerobic growth of Saccharomyces cerevisiae. However the interactions between the broth components and the biological reactions influencing pH make necessary a rigorous analysis of these phenomena in order to use correctly pH measurements. This problem has already be pointed out by San [1].

Keywords

Ammonia Solution Aerobic Growth Acetic Fermentation Neutralization Reagent AcetiC Acid Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    San K.Y., Stephanopoulos G., Biotechnol. Bioeng., 26,1209–1218 (1984).CrossRefGoogle Scholar
  2. [2]
    Pons MN., Grefte JL, Bordet J., Talanta, 30, 3 205–208 (1983)Google Scholar
  3. [3]
    Bar R., Gainer JL., Kirwan DJ., Biotechnol. Bioeng., 29, 796–798 (1987). CrossRefGoogle Scholar
  4. [4]
    Garrido-Sanchez LE., Dantigny P., Pons MN., Biotechnol. Techniques, 2,1,17–22 (1988). Google Scholar

Copyright information

© Society of Chemical Industry 1989

Authors and Affiliations

  • M. N. Pons
    • 1
  • L. Garrido-Sanchez
    • 1
  • J. M. Engasser
    • 1
  1. 1.Laboratoire des Sciences du Génie ChimiqueCNRS-ENSIC-INPLNancy CedexFrance

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