Abstract
Polysomes prestained with low concentrations of Coomassie blue gave rise to visible bands representing aggregates of different sizes when centrifuged in a sucrose density gradient and showed profiles similar to unstained polysomes. The visibility of the bands rendered them easy to recuperate and analyze. The stained polysomes were more active than the unstained specimens in protein synthesis, including the synthesis of tissue specific proteins in vitro. This enhanced activity was abolished when messenger RNAs were isolated from the prestained polysomes and translated in vitro. It is suggested that Coomassie blue may exert its influence by acting on one or more of the protein components involved in stabilizing polysomal structure and function.
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Ven Murthy, M.R., Levesque, G., Bharucha, A.D., Charbonneau, R., Viallard, JL., Dastugue, B. (1989). Influence of Dye Binding on the Integrity and Function of Nucleic Acids and Nucleoproteins: Complexation of Polysomes with Coomassie Blue. In: Vijayalakshmi, M.A., Bertrand, O. (eds) Protein-Dye Interactions: Developments and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1107-9_31
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DOI: https://doi.org/10.1007/978-94-009-1107-9_31
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6989-2
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