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Affinity Precipitation of (NAD Dependent Dehydrogenase) Lactate Dehydrogenase Using Cibacron Blue Dimers

  • B. Riahi
  • M. A. Vijayalakshmi

Summary

The selective precipitation of lactate dehydrogenase, by using two structural variants of the Cibacron Blue dimers is demonstrated. While both the dimers are showing good selectivity, the dimer having a hexamethylene group as the bridge is more effective, both in terms of purification fold and yield. Thus, this dimer with hexamethylene bridge is able to precipitate selectively the lactate dehydrogenase from a crude extract of rabbit muscle with 110 fold purification, and 4750% yield, while a second dimer having a diaryl group gives a 90 fold purification with 2500% yield from the same extract. The purified enzyme shows a single band in the SDS-PAGE analysis.

Keywords

Crude Extract Lactate Dehydrogenase Dimer Versus Rabbit Muscle Fold Purification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Elsevier Science Publishers LTD 1989

Authors and Affiliations

  • B. Riahi
    • 1
  • M. A. Vijayalakshmi
    • 1
  1. 1.Laboratoire de Technologie des SéparationsUniv. Technol. CompiègneCompiègneFrance

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