The Use of Biospecific Elution in Purifying Proteins from Dye-Ligand Adsorbents
Elution of protein from dye-ligand adsorbents can be carried out by non-specific means (salt or pH changes), in which case generally poor resolution is achieved. Biospecific elution using natural ligands to the protein can, in the appropriate conditions, achieve a much higher degree of purification. For biospecific (affinity) elution to succeed, the dye must bind at the natural ligand’s site; screening of a range of dyes to find such an interaction may be necessary to achieve optimum results from affinity elution. The use of differential column adsorption with affinity elution can result in a one-step purification of 20 to 100-fold at the first step in many instances.
KeywordsNatural Ligand FEBS Letter Phosphoglycerate Mutase Ionic Strength Range Cibacron Blue F3GA
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