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A ‘Blood Spot’ Androstenedione Radioimmunoassay Able to Detect Congenital Adrenal Hyperplasia

  • S. Thomson
  • A. M. Wallace
  • B. Cook

Abstract

Most neonatal screening programmes for congenital adrenal hyperplasia (CAH) are based on the measurement of 17-hydroxyprogesterone in blood eluted from filter paper samples (Pang et al., 1988). Androstenedione (A4) measurement may offer greater potential as a diagnostic aid because it should also detect the second most common form of CAH, 11β-hydroxylase deficiency. Unfortunately, most A4 immunoassays are too insensitive to allow large-scale neonatal screening for CAH using filter paper blood spot samples. We describe below a sensitive radioimmunoassay (RIA) able to measure A4 in blood spot samples with or without prior solvent extraction.

Keywords

Congenital Adrenal Hyperplasia Precocious Puberty Neonatal Screening Hydroxylase Deficiency Classical Congenital Adrenal Hyperplasia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Pang, S., Wallace, A. M., Hofman, L., Thuline, H., Dorche, C., Lyon, I. C., Dobbins, H., Kling, S., Fujieda, K. and Suwa, S. Worldwide experience in newborn screening for classical congenital adrenal hyperplasia due to 21-hydroxylase deficiency. Pediatrics 81 (1988) 866–874PubMedGoogle Scholar
  2. Thomson, S. Studies on a novel androstenedione radioimmunoassay and its application to the neonatal detection of congenital adrenal hyperplasia. Fellowship Thesis, 1986, Institute of Medical Laboratory SciencesGoogle Scholar
  3. Wallace, A. M., Beastall, G. H., Cook, B., Currie, A. J., Ross, A. M., Kennedy, R. and Girdwood, R. W. A. Neonatal screening for congenital adrenal hyperplasia: a programme based on a novel direct radioimmunoassay for 17-hydroxyprogesterone in blood spots. J. Endocrinol. 108 (1986) 299–308PubMedCrossRefGoogle Scholar

Copyright information

© SSIEM and Kluwer Academic Publishers 1989

Authors and Affiliations

  • S. Thomson
    • 1
  • A. M. Wallace
    • 2
  • B. Cook
    • 2
  1. 1.Neonatal Screening Unit, Department of BacteriologyStobhill General HospitalGlasgowUK
  2. 2.Institute of BiochemistryRoyal InfirmaryGlasgowUK

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