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Gas Chromatography of PQQ

  • O. Suzuki
  • T. Kumazawa
  • H. Seno
  • T. Matsumoto
  • T. Urakami

Abstract

A new method for gas chromatographic analysis of PQQ is presented. A sample was made weakly-alkaline and passed through a Sep-Pak C18 cartridge. The eluate was then made acid with HCl and passed through the second Sep-Pak C18 cartridge. Finally, PQQ was eluted with pyridine solution; it was evaporated to dryness. The residue was derivatized with a phenyltrimethylammonium reagent and subjected to gas chromatography (GC). The chemical structure of the reaction product was estimated to be 3-[1-methyl-3, 5-di(methoxycarbonyl)-pyrrole-2-yl]-2, 4, 6-tri(methoxycarbonyl)pyridine by mass spectral analysis. GC was made with SPB-1 fused silica wide-bore and narrow-bore capillary columns, and with flame ionization detection. The detection limit of PQQ was 5–10 ng in an injected volume with the narrow-bore capillary column in the splitless mode. The endogenous PQQ in human urine, plasma, brain and liver, and rat brain and liver, was below the detection limit (less than 100 ng/ml or g).

Keywords

Splitless Mode Lysyl Oxidase Nitrogen Flow Rate Resonance Raman Spectroscopy Pyrroloquinoline Quinone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • O. Suzuki
    • 1
  • T. Kumazawa
    • 1
  • H. Seno
    • 1
  • T. Matsumoto
    • 2
  • T. Urakami
    • 3
  1. 1.Department of Legal MedicineHamamatsu University School of MedicineJapan
  2. 2.First Department of SurgeryNagoya University School of MedicineNagoya 466Japan
  3. 3.Niigata Research LaboratoryMitsubishi Gas Chemical Company, Inc.NiigataJapan

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