Agrobacterium tumefaciens is capable of transferring a defined piece of DNA (T-DNA) containing tumorigenic loci from its tumor-inducing (Ti) plasmid into the genome of a large number of gymnosperms and angiosperms [1–3]. This process requires the cis acting T-DNA border sequences [4–6] and the trans acting virulence (vir) functions encoded by the Ti plasmid and the Agrobacterium chromosome [7–10]. The transfer process is fully active when the vir functions and the T-DNA are located on separate compatible replicons in Agrobacterium . These features made the development of binary vectors possible [12–15]. In such systems, the Agrobacterium host strain contains a wild-type Ti plasmid or disarmed (tumor genes deleted) Ti plasmid that carries the vir functions and serves a a helper. The T-DNA borders are located on a compatible replicón that will function in both E. coli and Agrobacterium. DNA that is inserted between the T-DNA borders will be efficiently transferred to and stably maintained within the plant genome.
KeywordsBinary Vector Fall Leaf Lake Callus Maintenance Medium Transform Tobacco Cell Transcription Termination Region
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