Abstract
The study of plant gene regulation has moved rapidly into the description of transcription regulatory proteins that bind to promoter or enhancer DNA [for example 1–5]. However, most of this work has been conducted in vitro and has been made possible by the gel retardation assay [6].
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References
Maier U-G, Brown JWS, Toloczyki C, Feix G (1987) Binding of a nuclear factor to a consensus sequence in the 5’ flanking region of zein genes from maize. EMBO J 6: 17–22.
Green P, Kay S, Chua N-H (1987) Sequence specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J 6: 2543–2549.
Mikami K, Tabata T, Kawata T, Nakayama T, Iwabuchi M (1987) Nuclear protein(s) binding to the conserved DNA hexameric sequence postulated to regulate transcription of wheat histone genes. FEBS Lett 223: 273–278.
Jofuku KD, Okamuro JK, Goldberg RB (1987) Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region. Nature 328: 734–737.
Jensen EO, Marcker KA, Schell J, Bruijn FJ (1988) Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghaemoglobin lbc3 5’ upstream region. EMBO J 7: 1265–1271.
Freid M, Crothers DM (1981) Equilibria and kinetics of lac repressor operator interaction by Polyacrylamide gel electrophoresis. Nucl Acids Res 9: 6505–6525.
Ferl RJ, Nick HN (1987) In vivo detection of regulatory factor binding sites in the 5’ flanking region of maize Adhl. J Biol Chem 262: 7947–7950.
Feri RJ, Laughner B (1988) In vivo detection of regulatory factor binding sites of Arabidopsis thaliana Adh. submitted.
Nick H, Gilbert W (1985) Detection in vivo of protein-DNA interactions within the lac Operon of Escherichia coll Nature 313: 795–798.
Maxam AM, Gilbert, W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Meth Enzymol 65: 499–560.
Ogata RT, Gilbert W (1978) An amino terminal fragment of lac repressor binds specifically to lac operator. Proc Natl Acad Sci USA 75: 5851–5854.
Ephrussi A, Church G, Tonegawa S, Gilbert W (1985) B lineage-specific interactions of an immunoglobulin enhancer with cellular factors in vivo. Science 227: 134–140.
Church G, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995.
Nick H, Bowen B, Ferl RJ, Gilbert W (1986) Detection of cytosine methylation in the maize alcohol dehydrogenase gene by genomic sequencing. Nature 319: 243–246.
Saluz HP, Jiricny J, Jost JP (1986) Genomic sequencing reveals a positive correlation between the kinetics of strand-specific DNA demethylation of the overlapping estradiol/glucocorticoid- receptor binding sites and the rate of avian vitellogenin mRNA synthesis. Proc Natl Acad Sci USA 83: 7167–7171.
Shure M, Wessler S, Fedoroff N (1983) Molecular identification and isolation of the waxy locus in maize. Cell 35: 225–233.
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© 1989 Kluwer Academic Publishers, Dordrecht
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Ferl, R.J. (1989). In vivo detection of protein-DNA interactions. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_22
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DOI: https://doi.org/10.1007/978-94-009-0951-9_22
Publisher Name: Springer, Dordrecht
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