Abstract
The domestication of the crown gall disease causing Agrobacterium tumefaciens Ti plasmid for use in stable introduction of DNAs into the genome of plants has produced a technology permitting unparalleled experimental and practical manipulation of the genetic constitution of plants. Modified Ti plasmids carrying native or modified plant genes have permitted detailed analysis of the sequences necessary for light [5, 11, 12] and tissue or developmental [13, 4, 16] regulation of plant gene expression. Protein sequences important for the internalization of proteins within plastids have been elucidated [17]. The Ti transformation system has become a tool for the study of virus host range, symptom production and replication [10, 15]. Recently, the Ti system has been used to introduce the maize Ac transposable element into tobacco to examine the process and regulation of transposition and for use as a potential gene tagging system [3].
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References
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© 1989 Kluwer Academic Publishers, Dordrecht
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Rogers, S.G., Klee, H., Horsch, R.B., Fraley, R.T. (1989). Use of cointegrating Ti plasmid vectors. In: Gelvin, S.B., Schilperoort, R.A., Verma, D.P.S. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0951-9_2
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DOI: https://doi.org/10.1007/978-94-009-0951-9_2
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