In situ RNA hybridization in plant tissues
In situ hybridization is the most direct way of examining the modulation of gene expression during development at the individual cell level. This technique has been used primarily in animal tissues to determine the location of a particular mRNA or to map chromosomal positions of cloned DNA [2, 3, 9, 10, 24, 30]. This approach has also been used as a diagnostic tool for the detection of cells infected with viruses [6, 12, 19]. More recently, in situ hybridization has been applied to the localization of specific RNAs in plant tissues.
KeywordsAcetic Anhydride Antisense Transcript Sense Transcript Rubber Cement BEEM Capsule
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