Use of reporter genes to study gene expression in plant cells
The mechanism by which any organism controls the production of proteins by the differential expression of its genes has fascinated many researchers since protein production and gene expression were first linked. It has been shown that in most cases the production of polypeptides is mainly controlled at the level of gene transcription, although in some cases mRNA or protein stability might also play an important role. In many instances the detection of a polypeptide of interest, that may only be produced under certain conditions or in specific cell types within an organism, is rather complex or laborious since many of these gene products do not have easily detectable enzymatic or functional activities.
KeywordsNPTII Gene Neomycin Phosphotransferase Chloramphenicol Acetyl Transferase Chloramphenicol Acetyl Transferase Activity Chimaeric Gene
Unable to display preview. Download preview PDF.
- 3.Casadaban J, Martinez-Arias A, Shapira SK, Chou J (1983) In: Wu R, Grossman L, Moldave K (eds) β-Galactosidase gene fusions for analysing gene expression in Escherichia coli and yeast, Methods in Enzymology, Vol 100, pp 293–308. New York: Academic Press.Google Scholar
- 7.Gorman CM (1985) High efficiency gene transfer into mammalian cells. In: Glover DM (ed) DNA cloning, Vol II, pp 143–190.Google Scholar
- 11.Jones JDG, Dunsmuir P, Bedbrook J (1986) High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J 10:2411–2418.Google Scholar
- 14.Otten L, Schilperoort RA (1978) A rapid microscale method for the detection of lysopine and nopaline dehydrogenase activities. Biochim Biophys Acta 517:497–500.Google Scholar
- 18.Simpson J, Timko MP, Cashmore AR, Schell J, Van Montagu M, Herrera-Estrella L (1985) Light inducible and tissue specific expression of a chimaeric gene under control of the 5’ flanking sequence of a pea chlorophyl a/b binding protein gene. EMBO J 4:2733–2739.Google Scholar
- 19.Tempe J, Petit A (1982) Opine utilization by Agrobacterium. In: Kahl G, Schell J (eds), Molecular Biology of Plant Tumours, pp 451–459. New York: Academic Press.Google Scholar