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Studies of the Binding and Biological Actions of Ethidium

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Photochemical Probes in Biochemistry

Part of the book series: NATO ASI Series ((ASIC,volume 272))

Abstract

Photosensitive derivatives have been prepared corresponding to the biologically active analogs of the phenanthridine and acridine classes of DNA binding drugs. These were used as photoaffinity probes to study DNA binding, trypanocidal action, frameshift mutagenesis, mitochondrial mutagenesis, and DNA repair. In each instance there was good evidence that photoaffinity labeling provided covalent adducts corresponding to the noncovalent binding which led to the biological actions of the parent compounds, and the resulting covalent adduct formation resulted in significant enhancement of biological activity as confirmation. These studies illustrated the power of photoaffinity labeling for studying biological receptors of these important agents.

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Authors and Affiliations

  1. The University of Texas Medical Branch, Galveston, Texas, USA

    K. Lemone Yielding

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  1. K. Lemone Yielding
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Editors and Affiliations

  1. Department of Biochemistry B, University of Copenhagen The Panum Institute, Blegdamsvej 3c, 2200, Copenhagen, Denmark

    Peter E. Nielsen

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© 1989 Kluwer Academic Publishers

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Yielding, K.L. (1989). Studies of the Binding and Biological Actions of Ethidium. In: Nielsen, P.E. (eds) Photochemical Probes in Biochemistry. NATO ASI Series, vol 272. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0925-0_19

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  • DOI: https://doi.org/10.1007/978-94-009-0925-0_19

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6905-2

  • Online ISBN: 978-94-009-0925-0

  • eBook Packages: Springer Book Archive

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