Synthetic reagents which cleave DNA in light- induced reactions (“photonucleases”) are useful as tools in biochemistry and of potential interest in medicine. We have designed and tested a wide variety of new as well as known reagents as “photonucleases”, and in this paper we review some of the results. Photochemical modification of DNA with azidoacridines, azidobenzamides, nitrobenzamides or 2-diazocyclopentadienylcarbonyloxy derivatives results in DNA cleavage, which is several fold increased upon subsequent piperidine treatment and which in some cases is A + G or G-specific.
KeywordsMinor Groove Uranyl Nitrate Natural Science Research Council Reagent Molecule Single Strand Nick
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