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Photonucleases

  • Ole Buchardt
  • Gunnar Karup
  • Michael Egholm
  • Troels Koch
  • Ulla Henriksen
  • Morten Meldal
  • Claus Jeppesen
  • Peter E. Nielsen
Part of the NATO ASI Series book series (ASIC, volume 272)

Abstract

Synthetic reagents which cleave DNA in light- induced reactions (“photonucleases”) are useful as tools in biochemistry and of potential interest in medicine. We have designed and tested a wide variety of new as well as known reagents as “photonucleases”, and in this paper we review some of the results. Photochemical modification of DNA with azidoacridines, azidobenzamides, nitrobenzamides or 2-diazocyclopentadienylcarbonyloxy derivatives results in DNA cleavage, which is several fold increased upon subsequent piperidine treatment and which in some cases is A + G or G-specific.

Keywords

Minor Groove Uranyl Nitrate Natural Science Research Council Reagent Molecule Single Strand Nick 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Ole Buchardt
    • 1
  • Gunnar Karup
    • 1
  • Michael Egholm
    • 1
  • Troels Koch
    • 1
  • Ulla Henriksen
    • 1
  • Morten Meldal
    • 1
  • Claus Jeppesen
    • 2
  • Peter E. Nielsen
    • 2
  1. 1.Research Center for Medical Biotechnology, The H. C. Ørsted InstituteUniversity of CopenhagenCopenhagen ØDenmark
  2. 2.Department of Biochemistry BThe Panum InstituteCopenhagenDenmark

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