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RecA-Directed Hybridization of Psoralen-Monoadducted DNA Oligonucleotides to Duplex Targets

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Photochemical Probes in Biochemistry

Part of the book series: NATO ASI Series ((ASIC,volume 272))

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Abstract

RecA protein can be used to introduce a psoralen-monoadducted DNA oligonucleotide into a duplex target site-specifically. Irradiation of the three-stranded DNA complex with long wavelength ultraviolet light results in formation of a site-specific crosslink between the inserted oligomer and its complement. Crosslinked complexes have been formed between pUC19 plasmid and modified oligomers ranging from 30 to 107 nucleotides in length. The efficiency of this two-step process depends upon several factors, including the nucleotide cofactor for RecA, the length of the oligomeric substrate, and the position of the psoralen adduct relative to the 5’-end of the oligomer. Absolute homology is not required.

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REFERENCES

  • Cimino GD, Gamper HB, Isaacs ST, and Hearst JE (1985). ‘Psoralens as photoactive probes of nucleic acid structure and function: organic chemistry, photochemistry, and biochemistry’ Ann Rev Biochem 54: 1151–1193.

    Article  PubMed  CAS  Google Scholar 

  • Cox MM and Lehman IR (1987).’Enzymes of general recombination’ Ann Rev Biochem 56:229–262.

    Article  PubMed  CAS  Google Scholar 

  • Gamper HB, Piette J, and Hearst JE (1984). ‘Efficient formation of a crosslinkable HMT monoadduct at the Kpnl recognition site’ Photochem Photobiol 40: 29–34.

    Article  PubMed  CAS  Google Scholar 

  • Gonda DK and Radding CM (1983). ‘By Searching Processively RecA Protein Pairs Molecules That Share a Limited Stretch of Homology’ Cell 34: 647–654.

    Article  PubMed  CAS  Google Scholar 

  • Honigberg SM, Gonda DK, Flory J, and Radding CM (1985). ‘The Pairing Activity of Stable Nucleoprotein Filaments Made from recA Protein, Single-stranded DNA, and Adenosine 5’-(γ-Thio)triphosphate’ J Biol Chem 260: 11845–11851.

    PubMed  CAS  Google Scholar 

  • Leahy MC and Radding CM (1986). ‘Topography of the Interaction of recA Protein with Single-stranded Deoxyoligonucleotides’ J Biol Chem 261: 6954–6960.

    PubMed  CAS  Google Scholar 

  • Muniyappa K, Shaner SL, Tsang SS, and Radding CM (1984). ‘Mechanism of the concerted action of recA protein and helix-destabilizing proteins in homologous recombination’ Proc Natl Acad Sci USA 81: 2757–2761.

    Article  PubMed  CAS  Google Scholar 

  • Radding CM (1982). ‘Homologous pairing and strand exchange in genetic recombination’ Ann Rev Genet 16: 405–437.

    Article  PubMed  CAS  Google Scholar 

  • Tsang SS, Muniyappa K, Azhderian E, Gonda DK, Radding CM, Flory J, and Chase JW (1985). ‘Intermediates in Homologous Pairing Promoted by recA protein’ J Mol Biol 185: 295–309.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of California, 94720, Berkeley, CA, USA

    Suzanne Cheng, Howard B. Gamper & John E. Hearst

  2. Division of Chemical Biodynamics, Lawrence Berkeley Laboratory, Berkeley, CA, 94720, USA

    Suzanne Cheng, Howard B. Gamper & John E. Hearst

  3. Microprobe Corporation, 1725 220th Street SE, No. 104, Bothel, WA, 98021, USA

    Howard B. Gamper

Authors
  1. Suzanne Cheng
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  2. Howard B. Gamper
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  3. John E. Hearst
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Editor information

Editors and Affiliations

  1. Department of Biochemistry B, University of Copenhagen The Panum Institute, Blegdamsvej 3c, 2200, Copenhagen, Denmark

    Peter E. Nielsen

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© 1989 Kluwer Academic Publishers

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Cheng, S., Gamper, H.B., Hearst, J.E. (1989). RecA-Directed Hybridization of Psoralen-Monoadducted DNA Oligonucleotides to Duplex Targets. In: Nielsen, P.E. (eds) Photochemical Probes in Biochemistry. NATO ASI Series, vol 272. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0925-0_12

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  • DOI: https://doi.org/10.1007/978-94-009-0925-0_12

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6905-2

  • Online ISBN: 978-94-009-0925-0

  • eBook Packages: Springer Book Archive

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