Abstract
Two experiments have been conducted, one in semi-solid Hoagland nutrient medium and the other in shallow pots containing saline soil. N2-fixing bacteria belonging to Azospirillum, Azotobacter, Klebsiella and Enterobacter were inoculated separately on kallar grass grown in semi-solid nutrient medium. It was shown that inoculation affects root proliferation and also results in 15N isotopic dilution. The % Ndfa ranged from 47–70 whereas no significant effect on the total nitrogen uptake was observed. The bacterial colonization of the root surface and the presence of enteric bacteria inside the root hair cells is reported. In a soil pot experiment, non-N2-fixing Polypogon monspeliensis was used as a reference plant (control). A treatment receiving a high rate of nitrogen was also used as a non-N2-fixing control. 15N-labelled ammonium sulphate at 20 kg N ha-1 and 90 kg N ha-1 was used. The % Ndfa in the aerial parts of kallar grass was 12–15 when P. monspeliensis was used as reference plant whereas 37–39% Ndfa was estimated when the treatment receiving high nitrogen fertilizer was used as a non-N2-fixing control. These investigations revealed some problems of methodology which are discussed.
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© 1989 Kluwer Academic Publishers
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Malik, K.A., Bilal, R. (1989). Survival and colonization of inoculated bacteria in kallar grass rhizosphere and quantification of N2-fixation. In: Skinner, F.A., Boddey, R.M., Fendrik, I. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 35. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0889-5_34
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DOI: https://doi.org/10.1007/978-94-009-0889-5_34
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