Immobilized proteins as HPLC chiral stationary phases

  • I. W. Wainer


Proteins are high-molecular-weight polymers composed of chiral subunits (L-amino acids). These polymers play a number of different roles in a biological system including the complexation (or binding) of xenobiotic materials. As proteins are chiral polymers, it is not surprising that the binding of small enantiomeric molecules is often stereospecific. This is especially true for the serum proteins α1-acid glycoprotein (AGP) and albumin (SA).


Human Serum Albumin Chiral Stationary Phasis Anionic Modifier Tetrabutylammonium Bromide Precolumn Derivatization 
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© Chapman & Hall 1989

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  • I. W. Wainer

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