Automated Multi-Stage Chromatographic Systems for Production of Biotherapeutics
Systems for multi-stage chromatographic purification of biotechnologysourced therapeutic proteins have been designed and built using carmercially available components, modified where necessary. The systems operate as sterile self-contained units totally under computer control. The chemistries of the individual stages are integrated so that the product peak from one stage is loaded directly on to the next stage. Automatic CIP can be added and programmed as part of the process cycle. Two such systems are in operation: the first is for the purification of human growth hormone from a recombinant mammalian cell line; the second is for the purification of therapeutic monoclonal antibodies. Both systems have been used for the production of material for clinical trials.
KeywordsHuman Growth Hormone Cell Culture Supernatant Solenoid Valve Process Cycle Product Peak
Unable to display preview. Download preview PDF.
- 1.Daniels, A.I., Pettersson, N.T. and Scandella, C.J., An automated purification system for the production of recombinant human superoxide dismutase. First Conference on Advances in Purification of Recombinant Proteins, Interlaken, Switzerland, March 1989.Google Scholar