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Cloning and Expression as a Tool to Easily Purify a New Highly Thermostable Archaebacterial ß-Galactosidase

  • Marco Moracci
  • Mario De Rosa
  • Mose’ Rossi

Abstract

Since enzymes from extremophiles are thermostable they could, in theory, be purified very easily after cloning and expression of their genes in mesophiles. This paper demonstrates that the gene of a new ß-galactosidase isolated from the extreme thermophile Sulfolobus solfataricus can be cloned and expressed in E. coli under the control of the archaebacterial promoter. The recombinant enzyme is similar to the native and, as expected, can be easily purified by heat treatment and isoelectric point precipitation. The partial purification and the properties of the recombinant enzyme are described.

Keywords

Recombinant Enzyme Native Enzyme Lactose Hydrolysis Thermophilic Enzyme Thermophilic Organism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© SCI 1990

Authors and Affiliations

  • Marco Moracci
    • 1
  • Mario De Rosa
    • 2
  • Mose’ Rossi
    • 1
  1. 1.Dipartimento di Chimica Organica e Biologica eIstituto di Biochimica delle Proteine ed Enzimologia, CNRItaly
  2. 2.Istituto di Biochimica delle MacromolecoleUniversità di NapoliItaly

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