Affinity Purification Using Radial Streaming Affinity Chromatography
A new support for affinity chromatography based on a cellulose-acrylic network was developed.
Using differently substituted supports, we have been able to purify trypsin, antibodies, and trypsinogen with their corresponding ligand. In a single pass, we were able to obtain good purity (> 85%)with good yields. Using coupled p-amino-benzamidine, we have shown that this purification method can be scaled-up very easily on radial-flow cartridges.
KeywordsRadial Flow Equilibration Buffer Good Purity Hydroxyl Derivative Membrane Base Material
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