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Affinity Purification Using Radial Streaming Affinity Chromatography

  • Y. Planques
  • H. Pora

Abstract

A new support for affinity chromatography based on a cellulose-acrylic network was developed.

Using differently substituted supports, we have been able to purify trypsin, antibodies, and trypsinogen with their corresponding ligand. In a single pass, we were able to obtain good purity (> 85%)with good yields. Using coupled p-amino-benzamidine, we have shown that this purification method can be scaled-up very easily on radial-flow cartridges.

Keywords

Radial Flow Equilibration Buffer Good Purity Hydroxyl Derivative Membrane Base Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© SCI 1990

Authors and Affiliations

  • Y. Planques
    • 1
  • H. Pora
    • 1
  1. 1.Cuno Europe/Life Sciences DivisionCergy-PontoiseFrance

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