Effect of pH on Polygalacturonase Adsorption in CM-Sephadex Gels

  • S. Harsa
  • D. L. Pyle
  • C. A. Zaror


This paper reports the effect of pH on the adsorption characteristics of fungal polygalacturonase in CM-Sephadex ion exchangers, in dilute solutions and in batch operations. The partition coefficient increases sharply at pH below 4.3, reaching a peak at about pH 3.2. Equilibrium isotherms follow the Langmuir law and are strongly affected by pH; the maximum adsorption capacity of adsorbent increases with decreasing pH. These findings reflect the effect of pH on the relative degrees of ionisation of adsorbate and adsorbent, and highlight the need to determine the optimum pH for every specific enzyme system.


Partition Coefficient Free Enzyme Maximum Adsorption Capacity Equilibrium Isotherm Adsorption Chromatography 
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Copyright information

© SCI 1990

Authors and Affiliations

  • S. Harsa
    • 1
  • D. L. Pyle
    • 1
  • C. A. Zaror
    • 1
  1. 1.Biotechnology Group Department of Food Science and technologyUniversity of ReadingReadingUK

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