Aqueous Two-phase Fractionation: Practical evaluation for productive biorecovery

  • J. G. Huddleston
  • K. W. Ottomar
  • D. Ngonyani
  • J. A. Flanagan
  • A. Lyddiatt


The recovery, by partitioning in aqueous two phase systems, of a range of potential bioproducts is considered. These include food functional protein and microbial enzymes fractionated from complex feedstocks and required in various states of purity. The influence of the basic parameters of aqueous two-phase systems: tie-line length, molecular weight and pH, upon partition of macromolecules is discussed in relation to molecular characteristics such as molecular weight, charge and hydrophobicity. The degree of purity attainable in limited numbers of discrete partitioning stages is addressed. Enhancement of resolution by adoption of approaches involving bioaffinity interactions is considered. Critical appraisal enables the current status of partitioning in aqueous two phase systems as applied to productive biotechnology to be considered.


Partition Coefficient Exclude Volume Effect Waste Yeast Single Stage Extraction Soluble Intracellular Protein 
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Copyright information

© SCI 1990

Authors and Affiliations

  • J. G. Huddleston
    • 1
  • K. W. Ottomar
    • 1
  • D. Ngonyani
    • 1
  • J. A. Flanagan
    • 1
  • A. Lyddiatt
    • 1
  1. 1.Biochemical Recovery Group, School of Chemical EngineeringUniversity of BirminghamEdgbaston, BirminghamUK

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