Cell Disruption and Removal of Insolubles

  • J. A. Asenjo


Disruption and separation of insolubles are unit operations used in the initial stages of product recovery/isolation. This paper reviews the main methods available both for cell disruption and removal of insolubles. Main insolubles considered are whole cells, cell debris and particles such as inclusion bodies. Separation and renaturation of inclusion bodies and other recombinant particles pose important challenges to modern process biotechnology. This paper critically assesses some of the main shortcomings of existing methodology used in the large scale today and points out recent novel developments that show potential for scale-up. Emphasis is given to methods that show improved selectivity and to novel process concepts in terms of their suitability for bio-processing.


Inclusion Body Cell Debris Fermentation Broth Cell Disruption Protein Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    .Asenjo, J.A. Selection of Operations in ’Separation Processes in Reparation Processes in Biotechnology’. Ed.: J.A. Asenjo, Marcel Dekker, N.Y., 1990, p3–16.Google Scholar
  2. 2.
    Asenjo, J.A. and Patrick, I. Large Scale Protein Purification in T ’Protein Purification Applications: a Practical Approach’. Eds.: E.L.V. Harris and S. Angal, IRL press, U.K., 1990, p1–29.Google Scholar
  3. 3.
    Hagen, A.J., Hatton, T.A. and Wang, D.I.C.Biotechnol. Bioeng., 1990, 35, 955–965.Google Scholar
  4. 4.
    Huang, R.-B. Ph.D. thesis, University of Reading, 1990.Google Scholar
  5. 5.
    Kula, M.R. Recovery Operations, in ’biotechnology’, Ed. H.J. Rehm and G. Reed, vol.2: Fundamentals of Biochemical Engineering, Verlag Chemie Mannheim, 1985.Google Scholar
  6. 6.
    Schutte, H. and Kula, M.R., Pilot and Process Scale Techniques for Cell Disruption. 32 International IUPAC Congress, Stockholm, 2–7 August, 1989.Google Scholar
  7. 7.
    Naglak, T.J., Hettwer, D.J. and Wang, H.Y., Chemical Permeabilization of Cells for Intracellular Product Release in ’Separation Processes in Biotechnology’. Ed. J.A. Asenjo, Marcel Dekker, N.Y., 1990, p.177–205.Google Scholar
  8. 8.
    Fish, N.M. and Lilly, M.D., Bio/Technology, 1984, 2, 623–627.CrossRefGoogle Scholar
  9. 9.
    Hettwer, D.J. and Wang, H.Y. in ’Reparation, Recovery and Purification in Biotechnology’. Ed.: J.A. Asenjo and J. Hong. ACS books, 1986, 314, p. 2-8.Google Scholar
  10. 10.
    Andrews, B.A. and Asenjo, J.A., Trends Biotech., 1987, 5, 273–277.CrossRefGoogle Scholar
  11. 11.Hunter, J.B. and Asenjo, J.A. in ’Separation, Recovery and Purification in Biotechnology’. Ed.: J.A. Asenjo and J. Hong, ACS books, 1986, 314, p. 9-31.Google Scholar
  12. 12.
    Asenjo, J.A., Andrews, B.A. and Pitts, J.M., Ann.N.Y.Acad. Sci., 1988, 542, 140–152.PubMedCrossRefGoogle Scholar
  13. 13.
    Buckland B.C., Richmond, W., Dunnill, P. and Lilly, M.D. in Industrial Aspects of Biochemistry. Ed: B. Spencer, Elsevier, Amsterdam, 1974, 30, 6 5–7 9.Google Scholar
  14. 14.
    Datar, R. and Rosen, C.-G., Downstream Process Economics in ’Separation Processes in Biotechnology’. Ed.: J.A. Asenjo, Marcel Dekker, N.Y., 1990, p.741–793.Google Scholar
  15. 15.
    Brocklebank, M.P. in Food Biotechnology, vol.1, ed.: R.D. King and P.S.J. Cheetham, Elsevier, Holland, 1987, 1, p139–192.CrossRefGoogle Scholar
  16. 16.
    Bjurstrom, E., Chem. Eng., 1985, 95,(4), 126–158.Google Scholar
  17. 17.
    Brown, D.E. and Kavanagh, P.R., Process Biochem., 1987, 22, 96–101.Google Scholar
  18. 18.
    Hedman, P., Intern. Biotechnol. Laborat., May/ June, 1984.Google Scholar
  19. 19.
    Andrews, B.A. and Asenjo, J.A., Aqueous Two-Phase Partitioning in ’Protein Purification Methods: a Practical Approach’. Eds. E.L.V. Harris and S. Angal, IRL Press, Oxford, 1989, p161–174.Google Scholar

Copyright information

© SCI 1990

Authors and Affiliations

  • J. A. Asenjo
    • 1
  1. 1.Biochemical Engineering LaboratoryUniversity of ReadingReadingEngland

Personalised recommendations