Reactive Solvent Extraction of Beta-Lactam Antibiotics
The reactive solvent extraction of olivanic acid MM13902, other beta-lactams and phenoxyacetic acid has been investigated. Extractants were quaternary nitrogen compounds or amine salts, for example tetrabutyl ammonium hydrogen sulphate, Amberlite LA2 hydrochloride, Alamine 336 hydrochloride and Aliquat 336. Solvents included dichloromethane and butyl acetate.
Under appropriate conditions, extractions in excess of 90% could be achieved with all compounds except amoxycillin. Back extraction could be brought about by nitrate and iodide ions, the latter being more effective but more expensive. With nitrate, several extractions were sometimes required to give satisfactory yields.
Small differences in lipophilicity and hence extracting power, for example between Alamine 336 hydrochloride and Aliquat 336, suggest the possibility of selective extraction.
KeywordsClavulanic Acid Sodium Nitrate Butyl Acetate Penicillin Versus Back Extraction
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- 1.Harris, T.A.J., Reuben, B.G., Cox, D.J., Vaid, A.K. and Carvell J., The cross-flow filtration of an unstable beta-lactam antibiotic fermentation broth. J, Chem. Tech. Biotechnol 1988, 42, 19–30.Google Scholar
- 2.Curbishley, P., Harris, T.A.J. and Reuben B.G., Cross-flow filtration of an unstable beta-lactam fermentation broth - the effect of antifoam. SCI Symposium on Membrane Reactors in Biotechnology, London, November 1989.Google Scholar
- 4.Part II, ibid, pages B11-B20 and other papers in this series.Google Scholar
- 5.Butterworth, D., Cole, M., Hanscomb, G. and Rolinson, G., Olivanic acids, a family of beta-lactam antibiotics with beta-lactamase inhibitory properties produced by Streptomyces species. J. Antibiotics. 1979, 32, 287–294.Google Scholar
- 6.Butterworth, D., Hood, J.D. and Verrall, M.S., The new beta-lactam antibiotics. In Advances in Biotechnological Processes. Vol l,eds. Mizrahi A. and Van Wezel A.L., A.R. Liss, New York, 1983, pp.251–292.Google Scholar