Receptor-mediated catabolism of LDL in rabbits fed cholesterol-free, semipurified diets containing casein or soy protein: a time-course study

  • S. Samman
  • P. Khosla
  • K. K. Carroll


Rabbits were fed semipurified diets containing casein or soy protein for 4 weeks. Increases in low density lipoprotein (LDL) cholesterol and protein were apparent within the first two weeks that the diets were fed and the changes were considerably greater in the rabbits fed casein. Receptor-mediated LDL apolipoprotein B (apo B) fractional catabolic rate (FCR) was decreased within 5 days of feeding the casein diet, prior to any significant change in plasma cholesterol. These studies indicate that down regulation of LDL receptors in vivo precedes the elevation in plasma cholesterol level induced by a casein diet.


Casein Diet Fractional Catabolic Rate Lipoprotein Protein 
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  1. Carroll, K.K. (1982) Hypercholesterolemia and atherosclerosis: Effects of dietary protein, Fed. Proc. 41, 2792–2796.PubMedGoogle Scholar
  2. Chao, Y.S., Yamin, T.T. and Alberts, A.W. (1982) Effect of cholestyramine on LDL binding sites to liver membranes from rabbits with endogenous hypercholesterolemia induced by a wheat-starch casein diet, J. Biol. Chem, 257, 3623–3627.PubMedGoogle Scholar
  3. Hrabek-Smith, J.M. and Carroll, K.K. (1987) A comparative study of serum lipoproteins in rabbits fed a natural ingredient or low-fat, cholesterol- free, semipurified diets containing casein or isolated soy protein, Biochem. Cell Biol. 65, 610–616.PubMedCrossRefGoogle Scholar
  4. Khosla, P., Samman, S. Carroll, K.K. and Huff, M.W. (1989a) Turnover of 125I-VLDL and 131I-LDL apolipoprotein B in rabbits fed diets containing casein or soy protein, Biochem. Biophys. Acta, 1002, 157–163.Google Scholar
  5. Khosla, P., Samman, S. and Carroll, K.K. (1989b) Receptor-mediated catabolism of LDL in rabbits fed casein or soy protein. FASEB J. 3, A957.Google Scholar
  6. Kita, T., Brown, M.S., Watanabe, Y. arid Goldstein, J.K. (1981) Deficiency of low density lipoprotein receptors on liver and adrenal gland of the WHHL rabbit, an animal model of familial hypercholesterolemia, Proc. Natl. Acad. Sei. USA, 78, 2268–2272.PubMedCrossRefGoogle Scholar
  7. Lovati M.R., Manzoni C., Canvavesi A. et al. (1987) Soybean protein diet increases low density lipoprotein receptor activity in mononuclear cells from hypercholesterolemic patients, J. Clin. Invest., 80, 1498–1502.PubMedCrossRefGoogle Scholar
  8. Matthew C.M.E. (1957) The theory of tracer experiments with 1311-labelled plasma proteins, Phys, Med. Biol., 2, 36–53.Google Scholar
  9. Redgrave T.G. (1984) Dietary proteins and atherosclerosis, Atherosclerosis, 52, 349–351.PubMedCrossRefGoogle Scholar
  10. Roberts, D.C.K., Stalmach M.E., Khalil M.W., Hutchinson J.C. and Carroll, K.K. (1981) Effects of dietary proteins on composition and turnover of apoproteins in plasma lipoproteins of rabbits, Can. J. Biochem, 56, 642–647.Google Scholar
  11. Samman, S., Khosla, P. and Carroll, K.K. (1989) Effects of dietary casein and soy protein on metabolism of radiolabelled low density apolipoprotein B in rabits, Lipids, 24, 169–172.PubMedCrossRefGoogle Scholar
  12. Terpstra, A.H.M., Harkes, L. and Van Der Veen, F.H. (1981) The effect of different proportions of casein in semipurified diets on the concentration of serum cholesterol and the lipoprotein composition in rabbits. Lipids, 16, 114–119.PubMedCrossRefGoogle Scholar
  13. Weisgraber, K.H., Innerarity, T.L. and Mahley, R.W. (1978) Role of the lysine residues in high affinity binding to cell surface receptors on human fibroblasts, J.Biol. Chem., 253, 9053–9062.PubMedGoogle Scholar
  14. Yamada, N., Shames, D.M., Stoudemire, J.B. and Havel, R.J. (1986) Metabolism of lipoproteins containing apolipoprotein B-100 in blood plasma of rabbits: heterogenity related to the presence of apolipoprotein E, Proc. Natl. Acad. Sci. USA, 83, 3479–3483.PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • S. Samman
    • 1
  • P. Khosla
    • 1
  • K. K. Carroll
    • 1
  1. 1.Department of BiochemistryUniversity of Western OntarioLondonCanada

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