Abstract
When micropropagating plants it is not always required that the final product in vitro is autotrophic. On the contrary, micropropagated roses with the lowest net photosynthesis yielded the best survival rate in the greenhouse, provided the culture conditions in the last stage of micropropagation were adequate. Sugar content, light intensity and controlled water retention capacity in the head space of the container are the most important factors. They can contribute to yield plants with a normal anatomy and physiology.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aoki, S., Oda, M. (1988) ‘Sensing of photosynthetic capacities of seedlings of lettuce with chlorophyll fluorescence’, Acta Hort. 230, 363 – 370.
Capellades, M. (1989) ‘Histological and ecophysiological study of the changes occurring during the acclimatization of in vitro cultured roses’, PhD dissertation, State University of Gent, Belgium, 98 pp.
Capellades, M., Garulla, C., Fontarnau, R. & Debergh, P. (1989) ‘The effect of environment on anatomy of stornata and epidermal cells in tissue cultured Rosa multiflora’, J. Amer. Soc. Hort. Sc., accepted for publication.
Capellades, M., Lemeur, R. & Debergh, P. (1989) ‘Effects of sugar content in the culture medium on starch accumulation in the chloroplasts and photosynthesis rate of tissue cultured roses’, Phys. Plant., submitted for publication.
Desjardins, Y., Laforge, F., Lussier, C. & Gosselin, A., (1988) ‘Effect of CO2 enrichment and high photosynthtic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants’, Acta Hort., 230, 45 – 53.
Fujiwara, K., Kozai, T. k Watanabe, I., (1987) ‘Fundamental studies on environment in plant tissue culture vessels. 3. Measurements of carbondioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets’, J. Agr. Met. 43, 21–30.
Grout, B.W.W. k Aston, M.J. (1977) ‘Transplanting of cauliflower plants regenerated from meristem culture. I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration’, Hort. Res., 17, 1 – 7.
Grout, B.W.W, k Price, F. (1987) ‘The establishment of photosynthetic independence in strawberry cultures prior to transplanting’, in G. Ducaté, Jacobs, M. k Simeon, A. (Eds.), Proc. Symp. Florizel 87, Plant Micropropagation in horticultural industries, Arlon, Belgium, pp. 55–60.
Grout, B.W.W. k Donkin, M.E. (1987) ‘Photosynthetic activity of cauli¬flower meristem cultures in vitro and at transplanting time’, Acta Hort., 212, 323–327.
Kautsky, H. k Franck, V. (1943) ‘Chlorophyll Fluoreszenz und Koh lensâureassimilà tion’, Biocem. Z., 315, 139–232.
Kozai, T., Iwanami, Y. k Fujiwara, F. (1987) ‘Environment control for masspropagation of tissue cultured plantlets. I. Effects of CO2 enrichment on the plant growth during the multiplication stage’, J. Agr. Met. 4, 22–26.
Kozai, T., Fujiwara, K. k Watanabe, I. (1986) ‘Fundamental studies on environments in plant tissue culture vessels. 2. Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers’, J. Agr. Met. 42, 119–127.
Langford, P.J. k Wainwright, H. (1987) ‘Influence of sucrose con-centration on the photosynthetic ability of in vitro grown rose shoots’, Acta Horticulturae, 22, 305–308.
Maene, L.J., (1985) Optimalisering van de overgang van weefsel- teeltplantjes naar in vivo omstandigheden’, PhD-thesis, State University Gent, Belgium, pp. 221.
Pinto Contreras, M. & Gaudillere, J.P. (1986) Plant Physiol. Biochem., 25 (1), 35–42.
Vanderschaeghe, A. & Debergh, P., (1987) ‘Technical aspects of the control af the relative humidity in tissue culture containers’, Med. Fac. Landbouww. Rijksuniv. Gent, 52(4), 1429–1437.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Kluwer Academic Publishers
About this chapter
Cite this chapter
Capellades, M., Vanderschaeghe, A., Lemeur, R., Debergh, P. (1990). How Important is Photosynthesis in Micropropagation?. In: Sangwan, R.S., Sangwan-Norreel, B.S. (eds) The Impact of Biotechnology on Agriculture. Current Plant Science and Biotechnology in Agriculture, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0587-0_3
Download citation
DOI: https://doi.org/10.1007/978-94-009-0587-0_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6752-2
Online ISBN: 978-94-009-0587-0
eBook Packages: Springer Book Archive