Cryopreservation of Plant Cell Cultures

The Importance of Pretreatments
  • U. Seitz
  • D. Banspach
  • E. Göldner
  • E. Reinhard
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 8)


Cryopreservation experiments were performed with cell suspension cultures of the following species: Atriplex hortensis L.,.A, litoralis L. (Chenopodiaceae), Berberis wilsoniae HEMSL. & WILS. (Berberidaceae), Coleus blumei BENTH. (Lamiaceae), Daucus carota L. (Apiaceae), Digitalis lanata EHRH. (Scrophulariaceae), and Panax ginseng C.A. MEY. (Araliaceae), The protocol comprises a preculture phase in nutrient media with enhanced osmolarity, treatment with cryoprotectants, slow freezing, storage at -196°C, rapid thawing, and post-thaw treatments. Biochemical capacities remain unchanged in frozen-thawed cultures. This was shown to be the case in D. carota, D. lanata, and P. ginseng. A survey on literature concerning this point is presented. Some alternatives to the standard cryopreservation procedure are given. Experiments are underway to investigate the physiological events during the preculture phase. As a consequence of osmotic stress cell viability decreased to a certain extent. A suspension of this process and even recovery have been observed in the cases of positive response. Further events such as the uptake of preculture additives and changes in the cytoplasm: vacuole volume ratio will be discussed.


Freeze Tolerance Rosmarinic Acid Panax Ginseng Plant Cell Culture Fluorescein Diacetate 
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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • U. Seitz
    • 1
  • D. Banspach
    • 1
  • E. Göldner
    • 2
  • E. Reinhard
    • 1
  1. 1.Pharmazeutisches InstitutUniversität TübingenTübingenGermany
  2. 2.Boehringer MannheimPenzbergGermany

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