Abstract
Cryopreservation experiments were performed with cell suspension cultures of the following species: Atriplex hortensis L.,.A, litoralis L. (Chenopodiaceae), Berberis wilsoniae HEMSL. & WILS. (Berberidaceae), Coleus blumei BENTH. (Lamiaceae), Daucus carota L. (Apiaceae), Digitalis lanata EHRH. (Scrophulariaceae), and Panax ginseng C.A. MEY. (Araliaceae), The protocol comprises a preculture phase in nutrient media with enhanced osmolarity, treatment with cryoprotectants, slow freezing, storage at -196°C, rapid thawing, and post-thaw treatments. Biochemical capacities remain unchanged in frozen-thawed cultures. This was shown to be the case in D. carota, D. lanata, and P. ginseng. A survey on literature concerning this point is presented. Some alternatives to the standard cryopreservation procedure are given. Experiments are underway to investigate the physiological events during the preculture phase. As a consequence of osmotic stress cell viability decreased to a certain extent. A suspension of this process and even recovery have been observed in the cases of positive response. Further events such as the uptake of preculture additives and changes in the cytoplasm: vacuole volume ratio will be discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bhandal, I.S., Hauptmann, R.M. and Widholm, J.M, (1985) Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells, Plant Physiol. 78, 430 – 432.
Boucaud, M-T. and Cambecedes, J. (1988) The use of 1,2-propandiol for cryopreservation of recalcitrant seeds: the model case ofZea mays imbibed seeds. Cryo-Lett. 9, 94–101.
Butenko, R.G., Popov, A.S., Volkova L.A., Chernyak, N.D. and Nosov, A.M. (1984) Recovery of cell cultures and their biosynthetic capacity after storage ofDioscorea deltoideaandPanax ginsengcells in liquid nitrogen, Plant Sci. Lett. 33, 285 – 292.
Camm, F.L. and Towers,G.H.N. (1977) Phenylalanin ammonia-lyase, Progr. Phytochem. 4, 169 – 188.
Chen, T.H.H., Kartha, K.K.,Leung, N.L. Kurz, W.G.W., Chatson, K.B. and Constabel, F. (1984) Cryopreservation of alkaloid-producing cell cultures of periwinkle (Catharanthus roseus), Plant Physiol. 75, 726 – 731.
Diettrich, B., Popov, A.S., Pfeiffer, B., Neumann, D., Butenko, R. and Luckner, M. (1982.) Cryopreservation ofDigitalis lanata cell cultures, Planta Med. 46, 82–87.
Dougall, D.K. and Whitten, G.H. (1980) The ability of wild carrot cell cultures to retain their capacity for anthocyanin synthesis after storage at -1400 C, Planta Med. Suppl. 129 – 135.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res. 50, 151 – 158.
Noè, W., Langebartels, C. and Seitz, H.U. (1980) Anthocyanin accumulation and PAL activity in a suspension culture ofDaucus carotaL., Planta 149, 283 – 287.
Pritchard, H.W., Grout, B.W.W., Short, K.C. and Reid, D.S. (1982) The effects of growth under water stress on the structure, metabolism and cryopreservation of cultured sycamore cells, in Franks, F. and Mathias, S.F. (eds.) The Biophysics of Water, Cichester, pp. 315 – 318.
Reuff, I. Seitz, U., Ulbrich, B. and Reinhard, E. (1988) Cryopreservation ofColeus blumeisuspension and callus cultures, J. Plant Physiol. 133, 414 – 418.
Seitz, U. (1987) Cryopreservation of plant cell cultures, Planta Med. 53, 311 – 314.
Seitz, U. and Reinhard, E. (1987) Growth and ginsenoside patterns of cryopreservedPanax ginsengcell cultures, J. Plant Physiol. 131, 215 – 223.
Seitz, U. Alfermann, A.W. and Reinhard, E. (1983) Stability of biotransformation capacity inDigitalis lanatacell cultures after cryogenic storage, Plant Cell Rep. 2, 273 – 276.
Seitz, U;., Reuff, I. and Reinhard, E. (1985) Cryopreservation of plant cell cultures, in Neumann, K.-H., Barz, W. and Reinhard, E. (eds.), Primary and Secondary Metabolism of Plant Cell Cultures, Springer, Berlin Heidelberg, pp. 323 – 333.
Watanabe, K., Mitsuda, H. and Yamada, Y. (1983) Retention of metabolic and differentiation potentials of greenLavandula veracallus after freeze preservation, Plant & Cell Physiol. 24, 119 – 122.
Widholm, J.M. (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells, Stain Technol. 47, 189 – 194.
Withers, L. A. (1985) Cryopreservation of cultured plant cells and protoplasts, in Kartha, K.K. (ed.), Cryopreservation of Plant Cells and Organs, CRC Press, Boca Raton, pp. 243 – 264.
Withers, L.A. and King, P.J. (1980) A simple freezing-unit and routine cryopreservation method for plant cell cultures, Cryo-Lett. 1, 213 – 220.
Ziebolz, B. and Forche, E. (1985) Cryopreservation of plant cells to retain special attributes, in Schaefer-Menuhr, A. (ed.), Advances in Agriculture and Biotechnology, Kluwer Acad. Pubi., Dordrecht, pp. 181.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Kluwer Academic Publishers
About this chapter
Cite this chapter
Seitz, U., Banspach, D., Göldner, E., Reinhard, E. (1990). Cryopreservation of Plant Cell Cultures. In: Sangwan, R.S., Sangwan-Norreel, B.S. (eds) The Impact of Biotechnology on Agriculture. Current Plant Science and Biotechnology in Agriculture, vol 8. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0587-0_28
Download citation
DOI: https://doi.org/10.1007/978-94-009-0587-0_28
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6752-2
Online ISBN: 978-94-009-0587-0
eBook Packages: Springer Book Archive