Sunflower Transformation: A Study of Selectable Markers
The overall utility of a transformation protocol depends to a large extent on the efficiency of the selection step. In the work presented here, we evaluated three selectable markers (kanamycin, paromomycin, and phosphinotricin) for their performance in sunflower transformation experiments, using hypocotyl expiants of three inbred lines and one hybrid. The assays were performed on several culture media currently in use for sunflower tissues.
The different sunflower genotypes showed a differential sensitivity depending on marker and medium used. Kanamycin did not show a significant inhibitory effect, while paromomycin and phosphinotricin proved to be efficient selective agents. More detailed studies revealed that the sensitivity towards a selectable marker is strongly influenced by factors such as the N-sources contained in the medium, as well as its hormonal balance.
KeywordsGlutamine Synthetase Gibberellic Acid Selectable Marker Casein Hydrolysate Helianthus Annuus
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