Microscopic Observations of Fusion Process of Rice and Lettuce Protoplasts

  • T. Taniguchi
  • T. Sato
  • K. Maeda
  • E. Maeda
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 8)


Protoplasts were isolated from rice callus and lettuce cotyledons. Their fusion process was studied by light and electron microscopy. Lettuce protoplast fusion could be easily produced by either of polyethylene glycol and electric methods. The transformation of fusion bodies during the fusion process was more easily observed by using the electric method than chemical one. After electric pulse application, the fusion of lettuce protoplasts was finished within about one hr, judging from their externals. On the other hand, the fusion of rice protoplasts occurred in a very short time after electric pulse application. Fusion was performed between lettuce protoplasts and rice protoplasts. Rice protoplasts contain no chloroplasts and lettuce protoplasts contain them. Therefore, both protoplasts could be easily distinguished under a light microscope. When the fusion between rice and lettuce protoplasts was induced by electric pulse application, chloroplasts in lettuce protoplasts moved into rice protoplasts within about one hr. Electron micrographs showed that the components from both protoplasts was located separately in the fusion bodies. Big rice fusion bodies produced by fusion of rice protoplasts contained some round-shaped particles. Their size was almost the same as that of the original protoplasts. These results indicate that it take a long time until the cytoplasm of rice protoplasts mix with the cytoplasm of lettuce protoplasts in the fusion body.


Electric Pulse Fusion Process Alginate Bead Protoplast Fusion Plant Protoplast 
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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • T. Taniguchi
    • 1
  • T. Sato
    • 1
  • K. Maeda
    • 1
  • E. Maeda
    • 1
  1. 1.Faculty of AgricultureNagoya UniversityNagoyaJapan

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