Immunogold Localization of Ribulose 1,5-Bisphosphate Carboxylase in Amphibious Eleocharis Species in Relation to C3 and C4 Photosynthesis

  • Osamu Ueno
  • Muneaki Samejima


The genus Eleocharis is a leaf-less sedge group growing in aquatic and wet environments. It includes some C4 species and possibly C3–C4 intermediate species, although most species of the genus are C3 (1). Recently, we found that Eleocharis vivipara, an amphibious plant, differentiated into the C4 type under the terrestrial conditions and into the C3 type under the submersed aquatic conditions (2). In order to elucidate the mechanisms of conversion of the photosynthetic metabolisms in E.vivipara, we attempt to study the inter- and intracellular localization of C3 and C4 photosynthetic enzymes. This study reports on the distribution of RuBP carboxylase/oxygenase (Rubisco) protein in the photosynthetic tissues of E.vivipara and E. baldwinii, another amphibious species which possesses the C4 characteristics at least under the terrestrial conditions.


Mesophyll Cell Terrestrial Condition Photosynthetic Metabolism IMMUNOGOLD Localization Terrestrial Form 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Osamu Ueno
    • 1
  • Muneaki Samejima
    • 1
  1. 1.Department of Applied PhysiologyNational Institute of Agrobiological ResourcesTsukuba Science City, Ibaraki 305Japan

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