Characterization of Site-Directed and Hybrid psbC Mutants of Synechocystis 6803

  • Shelly D. Carpenter
  • Jeroen Charite
  • Beth Eggers
  • Wim Vermaas


Photosystem II (PS II) is composed of at least five integral membrane proteins (together forming the core complex), in addition to several peripheral polypeptides. CP43 is a chlorophyll-binding protein in the core complex that is involved in the transfer of excitation energy to the reaction center, and it is encoded by the gene psbC. In this study, specific mutagenesis has been applied to alter psbC in the transformable, photoheterotrophic cyanobacterium, Synechocystis 6803. By transforming psbC deletion mutants with altered psbC gene sequences, modified psbC can be directly incorporated into the organism’s genome by homologous recombination (1). PS II defective mutants can be propagated photoheterotrophically on media containing an appropriate carbon source such as glucose (2–6). In this report we compile our recent results concerning two issues that have lately been the focus of our interest in CP43: the identification of the translation start codon, and the functional dissection of the protein. We have addressed these issues using site-directed mutagenesis and chimeric gene strategies, respectively.


Core Complex Translation Start Codon CP43 Protein Chlorophyll Basis Kanamycin Resistance Marker 
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  1. 1.
    Vermaas, W.F.J., Carpenter, S., and Bunch, C. (1989) in Phototsynthesis: Molecular Biology and Bioenergetics (Singhal, G.S., Barber, J., Dilley, R.A., Govindjee, Haselkorn, R. and Mohanty, P., eds.), pp. 21–35, Narosa, New Delhi.Google Scholar
  2. 2.
    Grigorieva, G. and Shestakov, S. (1982) FEMS Microbiol. Lett. 13,367–370.CrossRefGoogle Scholar
  3. 3.
    Williams, J.G.K. (1988) Meth. Enzymol. 167, 766–778.CrossRefGoogle Scholar
  4. 4.
    Golden, S.S., Brusslan, J. and Haselkorn, R. (1987) Meth. Enzymol. 153, 215–231.PubMedCrossRefGoogle Scholar
  5. 5.
    Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M. and Stanier, R.T. (1979) J. Gen. Microbiol. 111, 1–61.CrossRefGoogle Scholar
  6. 6.
    Carpenter, S.D. and Vermaas, W.F.J. (1989) Physiologia Plantarum 76, in press.Google Scholar
  7. 7.
    Vermaas, W.F.J., Williams, J.G.K. and Arntzen, C.J. (1987) Plant Mol. Biol. 8, 317–326.CrossRefGoogle Scholar
  8. 8.
    Berger, S.L. and Kimmel, A.R. (eds.) (1986) Guide to Molecular Cloning Techniques, Meth. Enzymol. 152.Google Scholar
  9. 9.
    Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) Meth. Enzymol. 145, 367–382.CrossRefGoogle Scholar
  10. 10.
    Vandeyar, M.A., Weiner, M.P., Hutton, C.J. and Batt, C.A. (1988) Gene 65, 129–133.PubMedCrossRefGoogle Scholar
  11. 11.
    Vermaas, W.F.J., Williams, J.G.K., Rutherford, A.W., Mathis, P. and Arntzen, C.J. (1986) Proc. Natl. Acad. Sci. USA 83, 9474–9477.PubMedCrossRefGoogle Scholar
  12. 12.
    Vermaas, W.F.J., Ikeuchi, M. and Inoue, Y. (1988) Photosynth. Res. 17, 97–113.CrossRefGoogle Scholar
  13. 13.
    Carpenter, S.D. and Vermaas, W.F.J. (1988) in Light Energy Transduction in Photosynthesis: higher plants and bacterial models (Stevens, S.E. Jr., and Bryant, D.A., eds.), pp. 327–331, Waverley Press, Baltimore.Google Scholar
  14. 14.
    Michel, H., Hunt, D.F., Shabanowitz, J. and Bennett, J. (1988) J. Biol. Chem. 263, 1123–1130.PubMedGoogle Scholar
  15. 15.
    Rochaix, J.D., Kuchka, M., Mayfield, S., Schirmer-Rahire, M., Girard-Bascou, J. and Bennoun,P. (1989) EMBO J. 8, 1013–1021.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Shelly D. Carpenter
    • 1
  • Jeroen Charite
    • 1
  • Beth Eggers
    • 1
  • Wim Vermaas
    • 1
  1. 1.Department of Botany and the Center for the Study of Early Events in PhotosynthesisArizona State UniversityTempeUSA

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