Advertisement

Expression of a Cyanobacterial Gene Regulated by CO2 Concentration

  • Dana Chamot
  • John R. Coleman

Abstract

Cyanobacteria, a large diverse group of photosynthetic prokaryotes, are known to possess an inducible inorganic carbon (Ci) concentrating mechanism which allows them to photosynthesize efficiently under the CO2 limitations of their environments (1). The accumulation of a large body of physiological evidence has led to the postulation that this mechanism is comprised of several components including a CO2 pump, a HCO3 pump, and the enzyme carbonic anhydrase (CA), which function in concert to supply Rubisco with saturating levels of CO2 when Ci levels in the external medium are limiting (2). This study explores the molecular biology of the cyanobacterial response to low Ci by attempting to clone and characterize the expression of a gene which appears to be regulated by CO2 concentration in the medium.

Keywords

Carbonic Anhydrase Anabaena Variabilis Enzyme Carbonic Anhydrase Photosynthetic Prokaryote Carbonic Anhydrase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aizawa, K. and Miyachi, S. (1986) FEMS Microbiol.Rev. 39, 215–233.CrossRefGoogle Scholar
  2. 2.
    Price, G. D. and Badger, M. R. (1989) Pl. Physiol. 89, 37–43.CrossRefGoogle Scholar
  3. 3.
    Allen, M. M. (1968) J. Phycol. 4, 1–4.CrossRefGoogle Scholar
  4. 4.
    Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982) Cold Spring Harbour Lab.Google Scholar
  5. 5.
    Marmur, J. (1961) J. Mol. Biol. 3, 208–218.CrossRefGoogle Scholar
  6. 6.
    Golden, S. S., Brusslan, J. and Haselkorn, R. (1987) Meth. Enzomol. 153, 215–237.CrossRefGoogle Scholar
  7. 7.
    Piccioni, R., Bellemare, G. and Chua, N. -H. (1982) in Methods in Chloroplast Molecular Biology (Edelman et al. eds), pp.985–1014, Elsevier, Amsterdam.Google Scholar
  8. 8.
    Bailly, J. and Coleman, J. R. (1988) Pl. Physiol. 87, 833–840.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Dana Chamot
    • 1
  • John R. Coleman
    • 1
  1. 1.Centre for Plant Biotechnology, Dept. of BotanyUniversity of TorontoCanada

Personalised recommendations