Molecular Models of 3-Dimensional Structures of Chloroplast and Cytoplasmic Phosphoglycerate Kinase from Wheat

  • Eileen M. McMorrow
  • Brian J. Sutton
  • J. William Bradbeer


The isoenzymes of phosphoglycerate kinase (PGK, EC from barley leaves have been purified in this laboratory and used to raise antisera (1). These antisera were used to isolate the wheat cDNAs, specific for each isoenzyme, which have since been sequenced (2). The derived amino acid sequences have been used to build models for the structures of both isoenzymes on an Evans and Sutherland graphics station. The co-ordinates obtained from the x-ray crystallographic studies of the yeast structure, which have been deposited in the Brookhaven protein data bank, have been used as a template (3).


Phosphoglycerate Kinase Chloroplast Sequence Side Chain Residue Derive Amino Acid Sequence Yeast Enzyme 
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  1. 1.
    McMorrow E.M. and Bradbeer J.W. (1989) Plant Physiol.(submitted).Google Scholar
  2. 2.
    Longstaff, M., Raines, CA., McMorrow, E.M., Bradbeer, J.W. and Dyer, T.A. (1989) Nucleic Acid Res. (in press).Google Scholar
  3. 3.
    Watson, H.C., Walker, N.P.C., Shaw, P.J., Bryant, T.N., Wendell, P.L., Fothergill, L.A., Perkins, R.E., Conroy,S.C., Dobson,M.J., Tuite, M.F., Kingsman,A.J. and Kingsman S.M.(1982) EMBO J. 1, 1635–1640.PubMedGoogle Scholar
  4. 4.
    Blake C.C.F., and Rice D.W. (1981) Phil. Trans. R.Soc. Lond. B, 293, 93–104.CrossRefGoogle Scholar
  5. 5.
    Sinev, M.A., Razgulyaev, O.I., Vas,M., TimchenKo, A.A., and Ptitsyn, O.B. (1989) Eur.J.Biochem. 180, 61–66.PubMedCrossRefGoogle Scholar
  6. 6.
    Mori,N., Singer-Sam,J. and Riggs, A.D. (1986) FEBS Lett., 204, 313–317.PubMedCrossRefGoogle Scholar
  7. 7.
    Littlechild, J.A., Davies,G.J., Gamblin, S.J. and Watson, H.C.(1987). FEBS Lett. 1, 123–126CrossRefGoogle Scholar
  8. 8.
    Gontero.B., Cardenas.M.L. and Ricard,J. (1988) Eur.J.Biochem.173, 437–443.PubMedCrossRefGoogle Scholar
  9. 9.
    Macioszek, J. and Anderson, L.E. (1987) Biochim. Biophys. Acta, 892, 185–190.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Eileen M. McMorrow
    • 1
  • Brian J. Sutton
    • 2
  • J. William Bradbeer
    • 1
  1. 1.Biosphere Sciences DivisionKing’s College LondonLondonUK
  2. 2.Biomolecular Sciences DivisionKing’s College LondonLondonUK

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