Light-Induced Accumulation of PS II Proteins and Expression of Oxygen Evolution Activity in Euglena
Analysis of biogenesis of oxygen evolution system in chloroplast is one of the promising ways for elucidating its structure and function. During the development of chloroplasts under illumination, the constituents of the oxygen evolution system have to be synthesized and organized properly in the thylakoid membrane to enable an efficient reaction. So far the process of assembly of the oxygen evolution system in thylakoid membrane has not been studied extensively, and information on this problem is quite limited. In the present study, we investigated the assembly of the PS II components in greening process of cells of Euglena gracilis Z mainly with SDS-PAGE and Western-blotting analysis. The results suggest that accumulation of the extrinsic 30 kDa protein in the thylakoid primarily determines the organization of the oxygen evolution system and expression of its activity in Euglena.
KeywordsThylakoid Membrane Oxygen Evolution Greening Process Euglena Gracilis White Fluorescent Lamp
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