The Glutamate 1-Semialdehyde Aminotransferase of Barley: Purification of the Protein, c-DNA Cloning, Sequencing and Characterisation of the mRNA
Chlorophyll synthesis in plants is regulated at steps converting glutamate into δ-aminolevulinate (ALA). The stromal protein glutamate 1-semialdehyde (GSA) aminotransferase catalyzes the last reaction in the synthesis of ALA. We identified and sequenced a c-DNA clone encoding the GSA-aminotransferase in order to study the expression of this enzyme and its catalytic mechanism of transamination.
KeywordsBarley Seedling Greening Barley Cyanogen Bromide Fragment Grown Barley Tigrina Mutant
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