The Glutamate 1-Semialdehyde Aminotransferase of Barley: Purification of the Protein, c-DNA Cloning, Sequencing and Characterisation of the mRNA

  • Bernhard Grimm
  • Simon P. Gough
  • C. Gamini Kannangara

Abstract

Chlorophyll synthesis in plants is regulated at steps converting glutamate into δ-aminolevulinate (ALA). The stromal protein glutamate 1-semialdehyde (GSA) aminotransferase catalyzes the last reaction in the synthesis of ALA. We identified and sequenced a c-DNA clone encoding the GSA-aminotransferase in order to study the expression of this enzyme and its catalytic mechanism of transamination.

Keywords

Barley Seedling Greening Barley Cyanogen Bromide Fragment Grown Barley Tigrina Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Bernhard Grimm
    • 1
  • Simon P. Gough
    • 1
  • C. Gamini Kannangara
    • 1
  1. 1.Department of PhysiologyCarlsberg LaboratoryCopenhagen ValbyDenmark

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