Chimeric Rescue of Rb. capsulatus Reaction Center Genes with Sequences from Rb. sphaeroides
Site-directed mutagenesis is becoming a very important technique in the study of photosynthetic reaction center structure-function relationships. However, there are certain disadvantages associated with this approach. First, it relies very heavily on the intuition of the investigator to select particular mutations that will give rise to interesting phenotypes; many times, mutagenesis can result in very little change in photosynthetic function, in complete loss of function, or in lack of assembly. Second, it is difficult to use specific mutagenesis to detect the concerted effect of several amino acids.
KeywordsReaction Center Delayed Fluorescence Trapping Time Interesting Phenotype Reaction Center Subunit
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