Chimeric Rescue of Rb. capsulatus Reaction Center Genes with Sequences from Rb. sphaeroides

  • Aileen K. Taguchi
  • Neal W. Woodbury
  • Jonathan W. Stocker
  • Steven G. Boxer

Abstract

Site-directed mutagenesis is becoming a very important technique in the study of photosynthetic reaction center structure-function relationships. However, there are certain disadvantages associated with this approach. First, it relies very heavily on the intuition of the investigator to select particular mutations that will give rise to interesting phenotypes; many times, mutagenesis can result in very little change in photosynthetic function, in complete loss of function, or in lack of assembly. Second, it is difficult to use specific mutagenesis to detect the concerted effect of several amino acids.

Keywords

Reaction Center Delayed Fluorescence Trapping Time Interesting Phenotype Reaction Center Subunit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Bibliography

  1. 1.
    Youvan, D.C., Ismail, S. and Bylina, E.J. (1985) Gene 38, 19–30PubMedCrossRefGoogle Scholar
  2. 2.
    Bylina, E.J., Ismail, S. and Youvan, D.C (1986) Plasmid 16, 175–181PubMedCrossRefGoogle Scholar
  3. 3.
    Williams, J.C., Steiner, L.A., Ogden, R.C., Simon, M.I. and Feher, G. (1983) Proc. Natl. Acad. Sci. USA 80, 6505–6509PubMedCrossRefGoogle Scholar
  4. 4.
    Sundstrom, V., van Grondelle, R., Bergstrom, H., Akesson, E. and Gilbro, T. (1986) Biochim. Biophys. Acta 851, 431–446CrossRefGoogle Scholar
  5. 5.
    Woodbury, N.W. and Parson, W.W. (1986) Biochim. Biophys. Acta 850, 197–210PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Aileen K. Taguchi
    • 1
  • Neal W. Woodbury
    • 1
  • Jonathan W. Stocker
    • 2
  • Steven G. Boxer
    • 2
  1. 1.Arizona State University and the Center for the Study of Early Events in PhotosynthesisTempeUSA
  2. 2.Department of ChemistryStanford UniversityStanfordUSA

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