Immunological Demonstration of Structural Differences of RuBP Carboxylase/Oxygenase in Mutants of Nicotiana tabacum
In Nicotiana tabacum mutants are known which differ with respect to their rates of photorespiration (1–6). Thus, wild type N.t. var. John William’s Broadleaf (JWB), the aurea mutant N.t. Su/su var. Aurea and the yellow-green phenotype of N.t. var. Consolation exhibit low rates of photorespiration. On the other hand the tobacco aurea mutant Su/su and N.t. var. Consolation green are the defective mutants which exhibit especially high rates of photorespiration. But also the yellow phenotype of N.t. var. Consolation shows on the average a higher photorespiration than the yellow-green form and the wild type. A higher oxygenase activity of the bifunctional stroma enzyme RuBP carboxylase/ oxygenase is responsible for this higher rates of photorespiration. Earlier serological studies on RuBP carboxylase/oxygenase of the different tobacco plants have shown that no correlation exists between a higher concentration of the enzyme and a higher photorespiratory activity (7). With one single antiserum to the wild type enzyme no differences with respect to the molecular structure between the enzymes of the mutants and the wild type enzyme can be detected (8).
KeywordsAntigenic Determinant Wild Type Enzyme RuBP Carboxylase Quantitative Binding Chloroplast Preparation
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