Photoreactivation and Photoinactivation of Photosystem II after a Complete Removal of Manganese from Pea Subchloroplast Particles
Photoinduced electron transfer and oxygen evolution are completely inhibited as a result of the complete (>98%) removal of Mn and water soluble proteins with m.w. 17,23 and 33 kD from pea photosystem II (PS-II) particles (DT-20) by means of I M tris-HCl (pH 8) and 0.5 M MgCl2/1–4/. Restoration of oxygen evolution after the treatment requires a joint addition of MnCl and CaCl2 /2–4/. Ca++ is highly specific in the reactivation (only Sr++ can replace it with a lower efficiency). At the same time, the restoration requires a very high concentration of Ca++ (10–20 mM) (Fig.1). The reactivation is observed in the absence of the proteins with m.w. 17,23 and.33 kD which are completely removed during extraction of Mn /3/ thus showing that all of them (including the 33 kD protein) are not the binding site for Mn and are not strictly necessary for oxygen evolution. When the sum of these proteins (isolated from DT-20 by I M CaCl2 /5/) is added to the particles, the reactivation is observed at much lower concentrations of Ca++ though the maximal rate of O2-evolution is not increased (Fig.1).
KeywordsActinic Light Donor Side Acid Fixation Redox Event Chlorophyll Fluorescence Yield
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