Functional Studies on the Reaction Pattern of the H+-ATP-Synthase in Spinach Chloroplasts

  • Frank Strelow
  • Tuan Tran-Anh
  • Bernd Rumberg


Steady state kinetics of both ATP synthesis and hydrolysis in dependence on all substrate species, i.e. ADP, ATP, phosphate, internal and external H+, have been measured and analysed. A functional model is derived which gives a unified description of both catalytical function and regulation of the enzyme. The main features of this model are:
  1. (1)

    Conformational changes in CFo induced by H+ translocation are strictly coupled to those in CF1 which switch over between loose and tight nucleotide binding (indirect coupling mechanism).

  2. (2)

    Conversion between ADP + Pj and ATP + H20 occurs spontaneously in the tightly bound state.

  3. (3)

    Regulation processes are integral constituents of the catalytic reaction cycle.

  4. (4)

    The H+/ATP coupling ratio is four.



Reaction Pattern Spinach Chloroplast Unify Description Steady State Kinetic Substrate Species 
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  1. [1]
    Rumberg, B., Schubert, K., Strelow, F., and Tran-Anh, T. (1989) this ProceedingsGoogle Scholar
  2. [2]
    Tran-Anh, T. and Rumberg, B (1987) in Progress in Photosynthesis Research Vol. III (J. Biggins, ed.) pp. 185–188, Martinus Nijhoff Publishers, DordrechtGoogle Scholar
  3. [3]
    Boyer, P.D. and Kohlbrenner, W.E. (1981) in Energy Coupling in Photosynthesis (Selman, B.R. and Selman-Reimer, S., eds.) pp. 231–340, Elsevier, AmsterdamGoogle Scholar
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    Penefsky, H.S. (1985) J. Biol. Chem. 260 13735–13741PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Frank Strelow
    • 1
  • Tuan Tran-Anh
    • 1
  • Bernd Rumberg
    • 1
  1. 1.Max-Volmer-InstitutTechnische Universität BerlinBerlin 12Germany

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