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Functional Studies on the Reaction Pattern of the H+-ATP-Synthase in Spinach Chloroplasts

  • Frank Strelow
  • Tuan Tran-Anh
  • Bernd Rumberg

Summary

Steady state kinetics of both ATP synthesis and hydrolysis in dependence on all substrate species, i.e. ADP, ATP, phosphate, internal and external H+, have been measured and analysed. A functional model is derived which gives a unified description of both catalytical function and regulation of the enzyme. The main features of this model are:
  1. (1)

    Conformational changes in CFo induced by H+ translocation are strictly coupled to those in CF1 which switch over between loose and tight nucleotide binding (indirect coupling mechanism).

     
  2. (2)

    Conversion between ADP + Pj and ATP + H20 occurs spontaneously in the tightly bound state.

     
  3. (3)

    Regulation processes are integral constituents of the catalytic reaction cycle.

     
  4. (4)

    The H+/ATP coupling ratio is four.

     

Keywords

Reaction Pattern Spinach Chloroplast Unify Description Steady State Kinetic Substrate Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature

  1. [1]
    Rumberg, B., Schubert, K., Strelow, F., and Tran-Anh, T. (1989) this ProceedingsGoogle Scholar
  2. [2]
    Tran-Anh, T. and Rumberg, B (1987) in Progress in Photosynthesis Research Vol. III (J. Biggins, ed.) pp. 185–188, Martinus Nijhoff Publishers, DordrechtGoogle Scholar
  3. [3]
    Boyer, P.D. and Kohlbrenner, W.E. (1981) in Energy Coupling in Photosynthesis (Selman, B.R. and Selman-Reimer, S., eds.) pp. 231–340, Elsevier, AmsterdamGoogle Scholar
  4. [4]
    Penefsky, H.S. (1985) J. Biol. Chem. 260 13735–13741PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Frank Strelow
    • 1
  • Tuan Tran-Anh
    • 1
  • Bernd Rumberg
    • 1
  1. 1.Max-Volmer-InstitutTechnische Universität BerlinBerlin 12Germany

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