Characterization of Nucleotide Binding Sites on Isolated Chloroplast ATPase by Modification with 7-Chloro-4-Nitro-Benzofurazan
The nucleotide binding sites on CF1 have been widely studied. Catalytic sites as well as “tight” binding sites are found to be located on the β-subunits of CF1 or between α- and β-subunits (1). Binding of ADP (ATP) involves two recognition areas: One on the phosphate chain (2) and the other one on the base moiety, which includes the C2, N1, C6-region of the base (3). Essential residues like the aminogroup of lysine (4) or arginine take part in recognition of the negatively charged Pα of the phosphate chain. The hydroxyl residue of tyrosine most probably forms an H-bridge to the base N1.
KeywordsSlow Phase Nucleotide Binding Site Base Moiety Phosphate Chain Essential Residue
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