pH-Dependent Changes in ATP and ADP Affinity for the Tight Nucleotide-Binding Site of the Chloroplast Coupling Factor CF1

  • A. N. Malyan
  • O. I. Vitseva


The ATPase complex of chloroplasts (H+-ATPase, ATP-synthase) carries out synthesis (hydrolysis) of ATP coupled with transmembrane transport of hydrogen ions. This complex consists of a hydrophytic catalytic part called coupling factor CF1, and a hydrophobic part, CF0, the function of which is to translocate protons towards CF1. CF1 can bind as many as six nucleotide molecules /1/. After CF1 precipitation by ammonium sulfate with subsequent gel filtration, the enzyme retains about 1 mol of tightly bound nucleotides consisting mainly of ADP /2/.


Hydrophobic Part Transmembrane Transport ATPase Complex Free Nucleotide Chloroplast Coupling Factor 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • A. N. Malyan
    • 1
  • O. I. Vitseva
    • 1
  1. 1.Inst. of Soil Science and PhotosynthesisAcad. Sci. USSRPushchino, Moscow RegionUSSR

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