pH-Dependent Changes in ATP and ADP Affinity for the Tight Nucleotide-Binding Site of the Chloroplast Coupling Factor CF1
The ATPase complex of chloroplasts (H+-ATPase, ATP-synthase) carries out synthesis (hydrolysis) of ATP coupled with transmembrane transport of hydrogen ions. This complex consists of a hydrophytic catalytic part called coupling factor CF1, and a hydrophobic part, CF0, the function of which is to translocate protons towards CF1. CF1 can bind as many as six nucleotide molecules /1/. After CF1 precipitation by ammonium sulfate with subsequent gel filtration, the enzyme retains about 1 mol of tightly bound nucleotides consisting mainly of ADP /2/.
KeywordsHydrophobic Part Transmembrane Transport ATPase Complex Free Nucleotide Chloroplast Coupling Factor
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