Uni-Site Catalysis by the Reconstituted ATP Synthase from Chloroplasts
The ATP Synthase from chloroplasts catalyzes ATP synthesis/hydrolysis coupled with a transmembrane proton transport. The enzyme contains several nucleotide binding sites and it is assumed that three of them (located on the β subunits) have catalytic properties (1,2). The presence of several catalytic sites raises immediately the question whether they work independently or cooperatively.
KeywordsCatalytic Site Protonation State Nucleotide Binding Site Sequential Mechanism Centrifugation Column
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- 2.Girault, G., Beyer, G. and Galmiche, J. M. (1988) 5th EBEC report 247Google Scholar