Uni-Site Catalysis by the Reconstituted ATP Synthase from Chloroplasts

  • Peter Gräber
  • Petra Fromme
  • Ulrike Junesch


The ATP Synthase from chloroplasts catalyzes ATP synthesis/hydrolysis coupled with a transmembrane proton transport. The enzyme contains several nucleotide binding sites and it is assumed that three of them (located on the β subunits) have catalytic properties (1,2). The presence of several catalytic sites raises immediately the question whether they work independently or cooperatively.


Catalytic Site Protonation State Nucleotide Binding Site Sequential Mechanism Centrifugation Column 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Peter Gräber
    • 1
  • Petra Fromme
    • 2
  • Ulrike Junesch
    • 2
  1. 1.Biologisches InstitutUniversität StuttgartStuttgart 80Germany
  2. 2.Max-Volmer-InstitutTechnische Universität BerlinBerlin 12Germany

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