Surface-Enhanced Resonance Raman Scattering (SERRS) Spectroscopy of Bacterial Membranes: The Flavoproteins
The importance of biological membranes for cellular function is unquestionable. Membranes provide the structural framework for anchoring proteins and liposulable cofactors. The membranes also provide for compartmentation and spatial separation of different parts of the cell. In bacteria these functions are supported by the cytoplasmic membrane which becomes highly invaginated when the oxygen tension in the growth medium decreases to certain limits. The invaginations, called chromatophores, contain the photosyn-thetic apparatus. Flavoproteins are essential components of photosynthetic and respiratory electron transfer systems. We used SERRS spectroscopy to study the location of flavoproteins on the membrane of R. rubrum. The unique feature of SERRS spectroscopy is the fact that the resonance Raman signal from a scattering molecule is greatly enhenced when the molecule is on or close to the surface of a potentiostated electrode (1). The effect is extremely distant sensitive.
KeywordsPhotosynthetic Apparatus Bacterial Membrane SERRS Spectrum Prosthetic Group Raman Shift
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