Classification by antenna size gives two classes of PSII in chloroplasts from higher plants.The main part of PSII is located in the grana in the form of PSIIα (1). We have previously shown that there are subclasses of PSIIα (PSIIα1, PSαII2, PSIIα3 etc) having different antenna sizes (2). Sonication of inside-out thylakoid vesicles — which originate from the grana partitions and harbour PSIIα — followed by phase partition separated vesicles into different populations of vesicles having different functional antenna size of PSIIα. The functional antenna size was determined from light saturation curves of the oxygen evolution with phenylparabenzoquinone (1) or ferricyanide (P.Å Albertsson and S.G. Yu, unpublished) as electron acceptors. We now report data on the heterogeneity of PSIIα obtained by two additional, independent methods, fluorescence spectroscopy and gel electrophoresis.
KeywordsVariable Fluorescence Antenna Size Phase Partition PSII Core Complex Fluorescence Induction Curve
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